| 湖北株H3N8亚型马流感病毒HA基因的序列测定及其HA蛋白遗传特性分析 |
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| 引用本文： | 黄文强,郭巍,赵立平,盛名巍,王宇,李红梅,相文华.湖北株H3N8亚型马流感病毒HA基因的序列测定及其HA蛋白遗传特性分析[J].中国兽医学报,2010,30(5). |
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| 作者姓名： | 黄文强 郭巍 赵立平 盛名巍 王宇 李红梅 相文华 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨,150001 |
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| 基金项目： | 黑龙江省自然科学基金重点项目(ZD200812) |
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| 摘 要： | 2008年从湖北省分离到1株H3N8亚型马流感病毒A/equine/Hubei/6/08。以2002年美国KENTUKY株为模板设计HA基因测序引物,进行RT-PCR,然后测定该分离株的HA基因核苷酸序列。经NCBI上Blast同源性比较发现,与A/equine/Newmarket/5/2003(H3N8)同源性较高为98.7%。HA蛋白遗传进化分析表明该毒株隶属于H3N8亚型马流感病毒中的美洲系福罗里达亚系。该株与OIE现在推荐的疫苗候选株A/equine/Kentuck-y/5/2002(H3N8)HA1蛋白氨基酸序列比对发现有3处氨基酸替换位点;与OIE以往推荐的疫苗候选株A/e-quine/Kentucky/1/1994(H3N8)比对发现有11处氨基酸替换位点。研究结果表明该分离株可作为中国研制马流感疫苗的候选株。
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| 关 键 词： | H3N8亚型马流感病毒 HA基因 马流感疫苗 |
Genetic characterization of an equine influenza virus isolated in middle area of China in 2008 |
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| HUANG Wen-qiang,GUO Wei,ZHAO Li-ping,SHENG Ming-wei,WANG Yu,LI Hong-mei,XIANG Wen-hua.Genetic characterization of an equine influenza virus isolated in middle area of China in 2008[J].Chinese Journal of Veterinary Science,2010,30(5). |
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| Authors: | HUANG Wen-qiang GUO Wei ZHAO Li-ping SHENG Ming-wei WANG Yu LI Hong-mei XIANG Wen-hua |
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| Institution: | HUANG Wen-qiang,GUO Wei,ZHAO Li-ping,SHENG Ming-wei,WANG Yu,LI Hong-mei,XIANG Wen-hua (State Key laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | A H3N8 sybtype equine influenza virus (EIV) strain was isolated from unvaccinated racehorses in middle area of China in 2008.After sequencing the haemagglutinin (HA) gene,alignment and phylogenetic analyses were carried out using the HA amino acid sequences.The results showed that the Hubei isolate and A/equine/Newmarket/5/2003(H3N8) had the highest sequence identity,which was identified as a member of the American lineage.Compared with the latest recommended American lineage vaccine prototype A/equine/Kent... |
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| Keywords: | H3N8 subtype equine influenza virus HA gene equine influenza vaccine |
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