不同能量摄入水平对围产期奶牛脂肪组织Leptin mRNA和HSL mRNA表达的影响

选用围产期健康奶牛30头随机均分为3组,其中组为对照组,组和组为试验组。Ⅰ、Ⅱ、Ⅲ组于产前28d开始分别饲喂中国奶牛饲养标准(2000)标准日粮(能量摄入100%组)、标准日粮增加20%日粮(能量摄入120%组)和标准日粮减少20%日粮(能量摄入80%组),产后各组奶牛均饲喂同一标准日粮至56 d。试验各组奶牛分别于产前28、14 d、产后1、14、28、56 d尾根部手术采取脂肪样品,采用荧光RT-PCR法对脂肪组织Leptin mRNA及HSL mRNA表达水平进行定量分析。结果表明,不同能量摄入水平显著影响脂肪组织Leptin mRNA和HSL mR-NA表达。低能饲喂明显提高了脂肪组织中Leptin mRNA表达,降低了HSL mRNA表达;高能饲喂明显降低了脂肪组织中Leptin mRNA表达,提高了HSL mRNA表达。

Effects of different energy intakes during the dry period on abundance of mRNA of adipose leptin, HSL in periparturient dairy cows

Thirty healthy periparturient cows were allocated randomly into three groups and fed 100% energy diet(nutrition needs of China Dairy Cow Raise Standard 2000),120% energy diet and 80% energy diet respectively,beginning at 28 d before parturition,.After calving,all the cows were offered the lactation ration and ad libitum until 56 d postpartum.The subcutaneous fat were taked on tail root at 28,14 d before parturition and 1,14,28 d,and 56 d after parturition.The levels of Leptin mRNA and HSL mRNA were detected by real time fluorescence quantitative RT-PCR.The result showed that different energy intakes during the dry period had a great influence on abundance of Leptin mRNA and HSL mRNA of adipose tissue.The abundance of adipose Leptin mRNA in the cows fed 80% energy diet was significantly higher than that of 100% energy diet and the abundance of HSL mRNA decreased,while abundance of adipose Leptin mRNA and HSL mRNA in the cows fed 120% energy diet was a opposite to that of 80% energy diet.