| PCV2衣壳蛋白羧基端基因在T4噬菌体表面的展示及ELISA方法的建立 |
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| 引用本文： | 王宪文,曹永长,马静云,谢青梅,毕英佐.PCV2衣壳蛋白羧基端基因在T4噬菌体表面的展示及ELISA方法的建立[J].中国兽医学报,2011,31(8):1103-1106. |
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| 作者姓名： | 王宪文 曹永长 马静云 谢青梅 毕英佐 |
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| 作者单位： | 1. 华南农业大学动物科学院,广东广州510642／河南科技学院动物科学院,河南新乡453003
2. 华南农业大学动物科学院,广东广州,510642 |
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| 摘 要： | 用PCR扩增猪圆环病毒Ⅱ型广东分离株的衣壳蛋白羧基端基因,将PCR产物连接到pR质粒,转化DH5α细胞,筛选阳性克隆进行PCR鉴定并测序后,转化E2菌,将重组E2菌与缺陷型噬菌体T4-Z1同源重组后,得到重组噬菌体,经SDS-PAGE和Western blotting分析,表明衣壳蛋白片段在噬菌体表面正确展示,表达的融...
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| 关 键 词： | 猪圆环病毒Ⅱ型 衣壳蛋白 展示 |
The establishment of ELISA assay with the purifild recombined T4 bacteriophage displaying of the carboxyl terminus fragment of capsid gene on porcine circovirus type 2 |
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| WANG Xian-wen,CAO Yong-chang,MA Jing-yun,XIE Qing-mei,BI Ying-zuo.The establishment of ELISA assay with the purifild recombined T4 bacteriophage displaying of the carboxyl terminus fragment of capsid gene on porcine circovirus type 2[J].Chinese Journal of Veterinary Science,2011,31(8):1103-1106. |
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| Authors: | WANG Xian-wen CAO Yong-chang MA Jing-yun XIE Qing-mei BI Ying-zuo |
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| Institution: | WANG Xian-wen1,2,CAO Yong-chang1,MA Jing-yun1,XIE Qing-mei1,BI Ying-zuo1(1.College of Animal Science,South China Agricultural University,Guangzhou 510642,China,2.College of Animal Science,Henan Science and Technology Institute,Xinxiang,Henan 453003,China) |
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| Abstract: | Based on the gene sequence of Cap gene,a pair of primers was designed and,a 384 bp fraction of the carboxyl end of the capsid protein gene was obtained by PCR amplification,which was then cloned into plasmid pR to yield an integrative plasmid pR-Cap.The integrative recombinant plasmid was used to transform E.coli E2 host bacteria,and then E2 containing the recombinant plasmid was used for homologous recombination with lysozyme gene-deficient T4,thus yielding a recombinant bacteriophage T4-Cap.When purified ... |
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| Keywords: | porcine circovirus type 2 capsid display |
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