| 小肠结肠耶氏菌毒性质粒基因文库的构建 |
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| 引用本文： | 黎诚耀,韩文瑜,盖欣,王世若.小肠结肠耶氏菌毒性质粒基因文库的构建[J].中国兽医学报,1991(1). |
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| 作者姓名： | 黎诚耀 韩文瑜 盖欣 王世若 |
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| 作者单位： | 兽医大学微生物教研室
(黎诚耀，韩文瑜，盖欣)，兽医大学微生物教研室(王世若) |
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| 摘 要： | 本研究以pAT153质粒为载体,构建了小肠结肠耶氏菌毒性质粒(pVYE)经限制性内切酶Bam HI和PstI消化产生的DNA片段的基因文库.实验克隆了8株(pYBI～8)pVYE的BamHI片段和6株(pYPI～6)pVYE的PstI片段的重组子.其中pYB4、pYB7、pYB8重组质粒中插入的pVYE—Bam HIDNA片段的分子量分别为8.7、3.8、20kb;pYP3、pYP5、pYP6重组质粒中插入的pVYE-Pst I DNA片段的分子量分别为4.5、3.0、7.0kb.本实验结果为进一步筛选和制备特异性基因探针奠定了基础.
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| 关 键 词： | 小肠结肠耶氏菌 毒性质粒 基因文库 基因探针 |
Construction of Gene Libraries for Virulence Plasmid of Yersinia enterocolitica |
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| Li Chengyao et al.Construction of Gene Libraries for Virulence Plasmid of Yersinia enterocolitica[J].Chinese Journal of Veterinary Science,1991(1). |
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| Authors: | Li Chengyao |
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| Abstract: | Gene Libraries of virulence plasmid DNA fragments of Y. enterocolitica (pVYE) digested by restriction enzyme Bam HI or Pst I were constructed with the cloning vector pAT 153 plasmid Of the recombinant clones, 8(pYB 1 - 8) fragments cut by Bam HI and 6( pYP 1 - 6) fragments by Pst I were introduced into E. coli HB 101.The molecular weights of pYB 4 , pYB 7,pYB 8 are 8.7kb, 3.8kb and 20kb and those of pYP 3,pYP 5,pYP 6 4.5 kb, 3.0 kb and 6.8 kb, respectively. The results of this study would establish the fundamental for screen and preparation of the specific gene probe of virulent Y. enterocolitica. |
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| Keywords: | Yersinia enterocolitica virulence plasmid gene library gene probe |
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