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通过病毒分离鉴定和基因检测首次发现虎流感
引用本文:夏咸柱,高玉伟,扈荣良,王立刚,刘丹,邹啸环,黄耕,贺文琦,王玮,苏伟林,刘文良.通过病毒分离鉴定和基因检测首次发现虎流感[J].中国兽医学报,2003,23(2):107-110.
作者姓名:夏咸柱  高玉伟  扈荣良  王立刚  刘丹  邹啸环  黄耕  贺文琦  王玮  苏伟林  刘文良
作者单位:1. 解放军军需大学军事兽医研究所,吉林,长春,130062
2. 黑龙江省东北虎林园,黑龙江,哈尔滨,150027
3. 解放军军需大学中心实验室,吉林,长春,130062
4. 解放军第211医院,黑龙江哈尔滨150027
摘    要:应用F81猫肾传代细胞,从高热、拒食和间有神经症状的死亡率病科中分离获得1株病毒,经形态学、理化学、生物学和血清学系统鉴定,证明为流感病毒。采用流感病毒核蛋白基因引物,对分离病毒及该虎病科进行RT-PCR扩增和序列分析,结果从分离病毒和虎病科中均扩增出与理论值大小相符的464bp基因片段;其序列与A、B、C3型流感病毒相比较,同源性分别为84.9%、35.0%、24.8%。由此说明,所分离病毒为A型流感病毒,命名为/蘧/哈尔滨(中国)/01/2002。用此分离病毒静脉接种于3月龄家猫3号,均出现与病虎相似的临床症状,其中1号耐过,2只死亡。死亡猫剖检变化与死虎相似,主要是呈肺炎病变,并可从病科中回收到所接种病毒。从此分离病毒为HA抗原,进行虎与猫血清的HI抗体检测,结果康复虎血清比其病初血清、康复猫血清比其接种前血清HI抗体均增高4倍以上,表明该病毒具有致病性,是引起该虎与试验猫发病甚至死亡的病原。

关 键 词:基因检测  A型流感病毒  分离  鉴定    流感
文章编号:1005-4545(2003)02-0107-04
修稿时间:2003年1月9日

The First Finding of Tiger Influenza by Virus Isolation and Specific Gene Am- plification
XIA Xian-zhu ,GAO Yu-wei ,HU Rong-liang ,WANG Li-gang ,LIU Dan ,ZOU Xiao-huan ,HUANG Geng ,HE Wen-qi ,WANG Wei ,SU Wei-lin ,LIU Wen-liang.The First Finding of Tiger Influenza by Virus Isolation and Specific Gene Am- plification[J].Chinese Journal of Veterinary Science,2003,23(2):107-110.
Authors:XIA Xian-zhu  GAO Yu-wei  HU Rong-liang  WANG Li-gang  LIU Dan  ZOU Xiao-huan  HUANG Geng  HE Wen-qi  WANG Wei  SU Wei-lin  LIU Wen-liang
Institution:XIA Xian-zhu 1,GAO Yu-wei 1,HU Rong-liang 1,WANG Li-gang 2,LIU Dan 2,ZOU Xiao-huan 3,HUANG Geng 1,HE Wen-qi 1,WANG Wei 2,SU Wei-lin 2,LIU Wen-liang 4
Abstract:An influenza virus-like virus isolate was obtained from the specimen of a tiger died of high fever,anorexia,and twitch.This virus was confirmed to be influenza virus by characteristics of its morphology,physiochemistry,biology and serology.Further analysis was performed by amplification of the specific nucleic acid of influenza virus nucleoprotein gene.A 464 bp cDNA fragment,being amplified from both the virus cell culture and the suspension of specimens,was compared with the published sequences of influenza virus type A,B,C with the homology of 84.9%,35.0%,24.8%,respectively,indicating that the virus is an influenza virus type A isolate.This virus has been designated as A/tiger/Harbin/01/2002.Three 3-month-old domestic cats were infected with the virus by intravenous inoculation.One of the infected cats survived the infection and the other two were seriously sick and eventually died of the infection.The ill cats presented similar clinical signs and pathological changes,characterized by pneumonia,to that of tigers.The inoculated virus could be reisolated from the lung.Specific antibodies against the virus were measured by hemaglutination inhibition test.The antibody titers of survived tigers and cats were over 4 times higher than that of the sick and uninfected ones,respectively,demonstrating that the virus is pathogenic and sometimes even fatal for tigers and cats.This is the first report that influenza virus types A exists in tigers.
Keywords:influenza virus type A  isolation and identify  tiger
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