| 内毒素对肝脏p53表达及Ca2+浓度的影响 |
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| 引用本文： | 李勇,高洪,严玉霖,杨桂梅,孔令青,刘海峰,彭洁.内毒素对肝脏p53表达及Ca2+浓度的影响[J].中国兽医学报,2012(6):881-883,888. |
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| 作者姓名： | 李勇 高洪 严玉霖 杨桂梅 孔令青 刘海峰 彭洁 |
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| 作者单位： | 云南农业大学动物科学技术学院;云南省药物研究所GLP中心;昆明医学院;云南农业职业技术学院 |
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| 基金项目： | 国家自然科学基金资助项目(30560113);云南省高端科技人才引进计划项目(2009CI125);云南省现代农业生猪产业技术体系建设项目(云财农[2009]171号) |
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| 摘 要： | 为探讨内毒素在致大鼠肝损伤过程中对肝脏p53表达及Ca2+浓度的影响,将48只SD大鼠随机分2组:Ⅰ组为NS对照组;Ⅱ组为ET致病组,2组大鼠分别于第3、4、8、12h每组各处死6只,采集肝脏分别用于p53表达的Western-blotting检测和Ca2+浓度的FCM检测。结果显示,Ⅱ组p53的表达明显高于Ⅰ组(P<0.01),且Ⅱ组一直呈上升趋势;Ⅱ组Ca2+的荧光指数明显高于Ⅰ组(P<0.01),且一直呈上升趋势。结果表明,ET能有效上调肝细胞p53蛋白的表达及Ca2+浓度。
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| 关 键 词： | 内毒素 肝脏 p53蛋白 Ca2+浓度 |
Effect of endotoxin on expression of p53 and concentration of Ca2+ in liver of rats |
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| LI Yong,GAO Hong,YAN Yu-lin,YANG Gui-mei,KONG Ling-qing,LIU Hai-feng,PENG Jie.Effect of endotoxin on expression of p53 and concentration of Ca2+ in liver of rats[J].Chinese Journal of Veterinary Science,2012(6):881-883,888. |
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| Authors: | LI Yong GAO Hong YAN Yu-lin YANG Gui-mei KONG Ling-qing LIU Hai-feng PENG Jie |
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| Institution: | 1.College of Animal Science and Technology,Yunnan Agriculture University,Kunming 650201,China;2.Yunnan Institute of Materia Medica,GLP Centre,Kunming 650111,China;3.Kunming Medical University,Kunming 650031,China;4.Yunnan Agricultural Occupation Technical College,Kunming 650212,China) |
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| Abstract: | To investigate the effect of endotoxin on expression of p53 and concentration of Ca2+ in the damaged liver of rats induced by ET.Forty-eight Sprague-Dawley rats were randomly divided into two groups:the normal saline controls group(groupⅠ)and ET treatment group(groupⅡ).at 3,4,8 and 12 h past-treatment liver samples were taken for testing the expression of p53 by Western-blotting and the concentration of Ca2+ by FCM.For the expression of p53 and the fluorescence index(FI) of Ca2+,groupⅡwas markedly higher than those of groupⅠ(P<0.01) with a rising trend.The results indicated that ET could up-regulate the p53 protein expression and the concentration of Ca2+of hepatocyte in rats. |
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| Keywords: | endotoxin liver p53 protein concentration of Ca2+ |
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