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从长春地区牛肉和猪肉中检出产vero毒素大肠杆菌O157:H7
引用本文:周志江,黄上缓,郑明光,郑昊,孙伟斌,张让堂,汪力亚,王丽,李景云.从长春地区牛肉和猪肉中检出产vero毒素大肠杆菌O157:H7[J].中国兽医学报,2000,20(2):152-155.
作者姓名:周志江  黄上缓  郑明光  郑昊  孙伟斌  张让堂  汪力亚  王丽  李景云
作者单位:1. 解放军军需大学,兽医学院,吉林,长春,130062
2. 解放军302医院,北京,100039
基金项目:国家自然科学基金资助项目
摘    要:用分离培养法、生化反应鉴定、胶乳凝集试验和聚合酶链式反应(PCR)对长春地区市售的40份牛肉和30份猪肉样品进行了调查,结果从2份牛肉(5%)和1份猪肉(3.3%)样品中检出了产vero毒素大肠杆菌O157:H7。

关 键 词:vero毒素  大肠杆菌  牛肉  猪肉  O157:H7  PCR
修稿时间:1998-10-05

Isolation of Verotoxin-producing Escherichia coli O157:H7 from Beef and Pork in Changchun
ZHOU Zhi-jiang,HUANG Shang-yuan,ZHENG Ming-guang,ZHENG Hao,SUN Wei-bin,ZHANG Rang-tang,WANG Li-ya,WANG Li,LI Jing-yun.Isolation of Verotoxin-producing Escherichia coli O157:H7 from Beef and Pork in Changchun[J].Chinese Journal of Veterinary Science,2000,20(2):152-155.
Authors:ZHOU Zhi-jiang  HUANG Shang-yuan  ZHENG Ming-guang  ZHENG Hao  SUN Wei-bin  ZHANG Rang-tang  WANG Li-ya  WANG Li  LI Jing-yun
Abstract:A tolal of 70 samples of retail beef and pork fro m Changchun, China were assayed for the presence of Escherichia coli serogro up O157∶H7 by a multiple procedure. The procedure involves several steps, inclu ding selective enrichment, sorbitol-MacConkey agar (SMAC) culture, biochemical profile, slide and tube agglutination test and polymerase chain reaction (PCR). E.coli O157∶H7 was isolated from 2(5%) of 40 beef and 1 (3.3%) of 30 pork s amples.
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