绵羊自然感染贝氏莫尼茨绦虫对小肠黏膜免疫相关细胞的数量变化

运用组织学和组织化学染色法,分别对自然感染贝氏莫尼茨绦虫的成年绵羊（感染组）与正常成年绵羊（对照组）的小肠各肠段的上皮内淋巴细胞、浆细胞、杯状细胞和嗜酸粒细胞的分布和数量变化进行了比较研究。结果显示：感染组小肠各段上皮内淋巴细胞、浆细胞、杯状细胞和嗜酸粒细胞数量均显著高于对照组,其中感染组十二指肠、空肠和回肠的上皮内淋巴细胞数量分别比对照组增加了169.11%、230.38%和233.42%（P〈0.01）;嗜酸粒细胞数量分别比对照组增加了116.78%、123.87%和164.51%（P〈0.01）;浆细胞数量分别比对照组增加了127.34%、72.97%和328.26%（P〈0.01）;杯状细胞数量分别比对照组增加了33.40%、41.42%和133.17%。对照组和感染组上皮内淋巴细胞和杯状细胞从十二指肠到回肠均逐渐减少,相反,对照组和感染组嗜酸粒细胞数量从十二指肠到回肠均逐渐增多,对照组小肠固有层浆细胞数量从十二指肠到空肠增加,空肠到回肠减少,感染组小肠固有层浆细胞数量从十二指肠到回肠均逐渐增多;对照组十二指肠、空肠、回肠的上皮内淋巴细胞、上皮内杯状细胞、浆细胞和嗜酸粒细胞均差异极显著（P〈0.01）;感染组十二指肠、空肠、回肠的上皮内杯状细胞、浆细胞和嗜酸粒细胞均差异极显著（P〈0.01）,感染组上皮内淋巴细胞空肠与回肠差异极显著（P〈0.01）,但十二指肠与空肠差异不显著（P〉0.05）。研究结果表明,贝氏莫尼茨绦虫感染成年绵羊后,成年绵羊通过特异性黏膜免疫细胞上皮内淋巴细胞增生加强细胞免疫水平,浆细胞增生加强体液免疫水平,同时还通过非特异性黏膜免疫细胞,嗜酸粒细胞和杯状细胞的增生进一步加强黏膜免疫水平以抵抗贝氏莫尼茨绦虫对绵羊的感染。可见绵羊可以通过黏膜免疫相关细胞增生加强局部免疫力以监视虫体免疫逃逸来抵抗寄生虫的感染。

Number changes of mucosal immunity-associated cells in small intestine of goats which naturally infected by Moniezia benedeni

In order to study the number changes of mucosal immunityassociated cells in small intestine of goats which naturally infected by Moniezia benedeni(contract group),the distribution and number of intraepithelial lymphocytes(IELs),plasma cells,goblet cells and eosinophile granulocytes(EOS)were investigated by the histological and histochemical methods,and then compared with control group.The results showed that the number of these four kinds of cells in the contracted group were all significantly higher than control group,and the increased rate of IELs was 169.11% in duodenum,230.38% in jejunum and 233.42% in ileum(P<0.01),the increased rate of EOS was 116.78% in duodenum,123.87% in jejunum and 164.51% in ileum(P<0.01),the increased rate of plasma cells was 127.34% in duodenum,72.97% in jejunum and 328.26% in ileum(P<0.01),the increased rate of goblet cells was 33.40% in duodenum,41.42% in jejunum and133.17% in ileum.In addition,the number of IELs and goblet cells all decreased substantially from duodenum to ileum in both contracted and control groups,otherwise,the number of EOS increased from duodenum to ileum in two groups,and the number of plasma cells increased from duodenum to ileum in contracted group only but increased from duodenum to jejunum and decreased from jejunum to ileum in control group.Besides,the number of these four kinds of cells had obvious differences in the all small intestine of control group(P<0.01);The number of goblet cells,plasma cells and EOS also had obvious differences in the all small intestine of contracted group(P<0.01),but the number of IELs only had difference in jejunum and ileum(P>0.05).The results suggested that the cellular and humoral immunity function were respectively increased trough the proliferation of IELs and plasma cells which were special mucosal immunity-associated cells,and also trough the proliferation of non-special mucosal immunity-associated cells(EOS and goblet cells)enhanced the anti-infection.It is thus clear that goat could strength the local immune function by the proliferation of relative cells to monitor the immune escape of parasite.