云南水牛mtDNA D-loop遗传多样性研究 Genetic Diversity of mtDNA D-loop Region in Yunnan Buffaloes期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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云南水牛mtDNA D-loop遗传多样性研究

引用本文：	亐开兴,金显栋,张继才,尹以昌,李红伟,和嘉荣,和占星,罗再仁,雷初朝,黄必志.云南水牛mtDNA D-loop遗传多样性研究[J].中国牛业科学,2020,46(6):1-4.

作者姓名：	亐开兴金显栋张继才尹以昌李红伟和嘉荣和占星罗再仁雷初朝黄必志

作者单位：	云南省草地动物科学研究院,昆明650212;德宏州畜牧站,云南德宏678400;红河州畜牧技术推广站,云南蒙自661100;云南省种畜繁育推广中心,昆明650212;盈江县农业农村局,云南盈江679300;西北农林科技大学动物科技学院,陕西杨凌712100

基金项目：	云南省中青年学术和技术带头人后备人才培养对象(2018HB045)；云南省应用基础研究面上项目(2007C130M)；国家肉牛牦牛产业技术体系(CARS-37)资助。

摘要：	目的] 探究云南3个水牛品种(德宏水牛、滇东南水牛、盐津水牛)的mtDNA D-loop区的遗传多样性与母系起源。方法]采用PCR扩增、测序及生物信息学方法。结果] 本研究共分析了215条云南水牛mtDNA D-loop全序列,检测到107个多态位点,定义了86种单倍型。结果表明,云南3个水牛品种的mtDNA遗传多样性非常丰富,其中,滇东南水牛的遗传多样性最高(Hd: 0.946±0.017,Pi: 0.0162±0.0018),盐津水牛的遗传多样性最低(Hd: 0.805±0.063,Pi: 0.0141±0.0031)。系统发育分析结果表明,在3个云南水牛品种中检测到2个主要支系(A和B支系及其亚支系)及一个稀有支系C,其中A支系为主要支系(79.07%),且经历了强烈的群体扩张。结论] 云南3个水牛品种具有丰富的mtDNA遗传多样性,主要有A与B两个母系起源。
关键词：	云南水牛 mtDNA D-loop 遗传多样性
收稿时间：	2020/8/17 0:00:00
修稿时间：	2020/8/25 0:00:00
Genetic Diversity of mtDNA D-loop Region in Yunnan Buffaloes

QU Kai-xing,JIN Xian-dong,ZHANG Ji-cai,YIN Yi-chang,LI Hong-wei,HE Jia-rong,HE Zhan-xing,LUO Zai-ren,LEI Chu-zhao and HUANG Bizhi.Genetic Diversity of mtDNA D-loop Region in Yunnan Buffaloes[J].China Cattle Science,2020,46(6):1-4.

Authors:	QU Kai-xing JIN Xian-dong ZHANG Ji-cai YIN Yi-chang LI Hong-wei HE Jia-rong HE Zhan-xing LUO Zai-ren LEI Chu-zhao and HUANG Bizhi

Institution:	Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,;AnimalSHusbandrySStationSofSDehongSPrefecture,Dehong,Yunnan,;Animal Husbandry Technology PromotionSStationSofSHongheSPrefecture,Mengzi,Yunnan,;Yunnan Breeding Stock and Breeding Promotion Center,Kunming,Yunnan,;Bureau ofSAgriculture and Rural Affairs of Yingjiang,Yingjiang,Yunnan,;College of Animal Science and Technology,Northwest A F University,Yangling,Shaanxi;Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,;AnimalSHusbandrySStationSofSDehongSPrefecture,Dehong,Yunnan,;Animal Husbandry Technology PromotionSStationSofSHongheSPrefecture,Mengzi,Yunnan,;Yunnan Breeding Stock and Breeding Promotion Center,Kunming,Yunnan,;Bureau ofSAgriculture and Rural Affairs of Yingjiang,Yingjiang,Yunnan,;College of Animal Science and Technology,Northwest A F University,Yangling,Shaanxi;Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,;AnimalSHusbandrySStationSofSDehongSPrefecture,Dehong,Yunnan,;Animal Husbandry Technology PromotionSStationSofSHongheSPrefecture,Mengzi,Yunnan,;Yunnan Breeding Stock and Breeding Promotion Center,Kunming,Yunnan,;Bureau ofSAgriculture and Rural Affairs of Yingjiang,Yingjiang,Yunnan,;College of Animal Science and Technology,Northwest A F University,Yangling,Shaanxi;Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,;AnimalSHusbandrySStationSofSDehongSPrefecture,Dehong,Yunnan,;Animal Husbandry Technology PromotionSStationSofSHongheSPrefecture,Mengzi,Yunnan,;Yunnan Breeding Stock and Breeding Promotion Center,Kunming,Yunnan,;Bureau ofSAgriculture and Rural Affairs of Yingjiang,Yingjiang,Yunnan,;College of Animal Science and Technology,Northwest A F University,Yangling,Shaanxi;Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,;AnimalSHusbandrySStationSofSDehongSPrefecture,Dehong,Yunnan,;Animal Husbandry Technology PromotionSStationSofSHongheSPrefecture,Mengzi,Yunnan,;Yunnan Breeding Stock and Breeding Promotion Center,Kunming,Yunnan,;Bureau ofSAgriculture and Rural Affairs of Yingjiang,Yingjiang,Yunnan,;College of Animal Science and Technology,Northwest A F University,Yangling,Shaanxi;Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,;AnimalSHusbandrySStationSofSDehongSPrefecture,Dehong,Yunnan,;Animal Husbandry Technology PromotionSStationSofSHongheSPrefecture,Mengzi,Yunnan,;Yunnan Breeding Stock and Breeding Promotion Center,Kunming,Yunnan,;Bureau ofSAgriculture and Rural Affairs of Yingjiang,Yingjiang,Yunnan,;College of Animal Science and Technology,Northwest A F University,Yangling,Shaanxi;Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,;AnimalSHusbandrySStationSofSDehongSPrefecture,Dehong,Yunnan,;Animal Husbandry Technology PromotionSStationSofSHongheSPrefecture,Mengzi,Yunnan,;Yunnan Breeding Stock and Breeding Promotion Center,Kunming,Yunnan,;Bureau ofSAgriculture and Rural Affairs of Yingjiang,Yingjiang,Yunnan,;College of Animal Science and Technology,Northwest A F University,Yangling,Shaanxi;Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,;AnimalSHusbandrySStationSofSDehongSPrefecture,Dehong,Yunnan,;Animal Husbandry Technology PromotionSStationSofSHongheSPrefecture,Mengzi,Yunnan,;Yunnan Breeding Stock and Breeding Promotion Center,Kunming,Yunnan,;Bureau ofSAgriculture and Rural Affairs of Yingjiang,Yingjiang,Yunnan,;College of Animal Science and Technology,Northwest A F University,Yangling,Shaanxi;Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan,Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan

Abstract:	Objective] The aim of the study was to explore the genetic diversity of three Yunnan buffalo breeds. Method] The complete mitochondrial DNA (mtDNA) D-loop regions of 215 buffaloes were analyzed using PCR sequencing and bioinformatics methods. Result] A total of 107 polymorphic sites were detected, which defined 86 haplotypes. Among the three buffalo breeds, the Diandongnan buffalo breed showed the highest genetic diversity (Hd: 0.946±0.017, Pi: 0.01620±0.00179). Our results showed that two main lineages (A and B with their sub-lineages) and one rare lineage (C) were detected in Yunnan buffaloes. The lineage A was extremely frequent in Yunnan buffaloes, and experienced a strong population expansion. Conclusion] The genetic diversity of these three Yunnan buffalo breeds were abundant, and mainly originated from two lineage A and B of swamp buffalo.

Keywords:	Yunnan buffalo mtDNA D-loop genetic diversity
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