体外成熟对牛卵母细胞孤雌激活后发育潜力的影响

本试验在卵母细胞体外成熟的研究基础上,研究了培养用水、卵泡液和成熟时间对卵母细胞体外成熟及孤雌激活后发育潜力的影响.结果表明(1)将卵母细胞分别于蒸馏水和Milli-Q超纯水配制的成熟培养液中培养22～24 h, 孤雌激活后, 卵裂率无显著差异(P>0.05); 但孤雌卵在超纯水配制的胚胎培养液中培养,囊胚发育率为22.3%, 明显高于在蒸馏水配制的培养液中的囊胚发育率14.1%(P<0.05).(2)在成熟培养液中添加10%、20%卵泡液,成熟卵母细胞孤雌激活后的囊胚发育率(32.3%, 30.9%)明显高于添加5%和0%卵泡液组的囊胚发育率(21.8%, 22.7%,P<0.05).卵母细胞在添加20%卵泡液的培养液中成熟培养,孤雌激活后囊胚孵化率明显低于添加0、5%、10%卵泡液组的囊胚孵化率(P<0.05).(3)成熟28 h或30 h的卵母细胞孤雌激活后,其囊胚发育率(30.5%或29.4%)明显高于成熟24 h或26 h组的囊胚发育率(20.5%或22.1%,P<0.05).

Effect of Oocyte Maturation in Vitro on Bovine Parthenogenetic Embryo Development Competence

Based on maturation in vitro of bovine oocytes, our research mainly focused on the effect of oocyte maturation in vitro on parthenogenetic embryos development competence in bovine. The results showed that The development rate to the blastocyst stage of oocytes activated after maturation for 22~24 h in medium prepared with Milli-Q water (29.7%) was significantly higher than with three-distilled water (14.1%, P<0.05). The addition of 10% or 20% BFF (bovine follicle fluid) in maturation medium obtained higher blastocyst rate (32.3% or 30.9%) than that in 5% or control treatment (21.8% or {22.7%}, P<0.05). In addition, the addition of 20% BFF in maturation medium inhibited the hatching of blastocyst. It suggested that oocytes matured for 28 h or 30 h before pathenogenetic activation resulted in higher blastocyte rate (30.5% or 29.4%) than those matured for 24 h or 26 h (20.5% or 22.1%, P<{0.05}).;