| 利用EST数据库资源克隆家蚕Bombyx mori6-磷酸葡萄糖酸脱氢酶基因 |
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| 引用本文： | 沈卫锋,牛宝龙,翁宏飚,孟智启.利用EST数据库资源克隆家蚕Bombyx mori6-磷酸葡萄糖酸脱氢酶基因[J].蚕桑通报,2005,36(1):14-19. |
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| 作者姓名： | 沈卫锋 牛宝龙 翁宏飚 孟智启 |
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| 作者单位： | 浙江省农业科学院蚕桑研究所,浙江,杭州,310021;浙江省农业科学院蚕桑研究所,浙江,杭州,310021;浙江省农业科学院蚕桑研究所,浙江,杭州,310021;浙江省农业科学院蚕桑研究所,浙江,杭州,310021 |
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| 摘 要： | 以果蝇6-磷酸葡萄糖酸脱氢酶(6-jpsjpg;icpmate dehydrogenase,6PGDH)基因cDNA序列为信息探针,对家蚕EST数据进行同源检索筛选,克隆到了家蚕6-磷酸葡萄糖酸脱氢酶基因的cD-NA序列,该基因全长2011bp.经RT-PCR克隆、序列分析验证,结果表明与电子克隆序列完全一致;该基因具有完整的开放阅读框(0RF),编码蛋白为483个氨基酸;通过与人、果蝇、家鼠、摇蚊、线虫的6PGDH蛋白序列比较,发现该基因具有高度的保守性.
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| 关 键 词： | EST数据库 RT-pCR 6-磷酸葡萄糖酸脱氢酶基因 家蚕 |
| 文章编号： | 2058-4069[2005]01-0014-06 |
Cloning 6-phosphogluconate Dehydrogenase (6PGDH)of the Silkworm Bombyx mori by Selecting EST Database |
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| SHEN Wei-feng,NIU Bao-long,WENG Hong-biao,MENG Zhi-qi.Cloning 6-phosphogluconate Dehydrogenase (6PGDH)of the Silkworm Bombyx mori by Selecting EST Database[J].Bulletin of Sericulture,2005,36(1):14-19. |
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| Authors: | SHEN Wei-feng NIU Bao-long WENG Hong-biao MENG Zhi-qi |
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| Abstract: | A novel silkworm(Bombyx mori) 6-phosphogluconate dehydrogenase (6PGDH) gene was cloned by blasting its EST database with the fruit fly homologous 6PGDH gene cDNA sequence information as a querying probe. It was also verified by RT-PCR. The full-length 6PGDH of 2011bp contains a complete open reading fragment (ORF: 117-1566) and encodes 248 Aa protein, which was very conservative in Homo sapiens, Drosophila melanogaster, Anopheles gambiae, Mus musculus, Caenorhabditis elegans. |
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| Keywords: | RT-pCR |
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