| 桑枝多糖的提取与纯化的试验研究 |
| |
| 引用本文： | 费建明,李玉峰,占鹏飞,冯建琴,朱祥瑞.桑枝多糖的提取与纯化的试验研究[J].蚕桑通报,2009,40(3):16-21. |
| |
| 作者姓名： | 费建明 李玉峰 占鹏飞 冯建琴 朱祥瑞 |
| |
| 作者单位： | 1. 浙江省湖州市农业科学研究院,浙江,湖州,313000
2. 浙江大学动物科学学院,浙江,杭州,310029 |
| |
| 基金项目： | 浙江省湖州市科技局重点项目 |
| |
| 摘 要： | 通过正交设计，用一般提取法对桑枝皮中多糖提取条件进行优化，并分别结合微波法提取、超声波提取法和酶解提取法，比较提取效果。将粗提液进行纯化，并比较乙醇沉淀法和大孔吸附树脂的纯化效果。结果：一般提取法中最适的提取条件组合为提取时间100min、溶液—材料比20倍、pH5，同时结合生物酶提取法可将提取率提高18．57％；用AB-8型大孔吸附树脂纯化，可获得纯度84．57％的多糖。
|
| 关 键 词： | 桑枝 多糖 提取 纯化 |
Experimental Research on Extraction and Purification of Ramulus mori Polysaccharide |
| |
| FEI Jian-ming,LI Yu-feng,ZHAN Peng-fei,FEN Jian-qing,ZHU Xiang-rui.Experimental Research on Extraction and Purification of Ramulus mori Polysaccharide[J].Bulletin of Sericulture,2009,40(3):16-21. |
| |
| Authors: | FEI Jian-ming LI Yu-feng ZHAN Peng-fei FEN Jian-qing ZHU Xiang-rui |
| |
| Institution: | FEI Jian-ming1,LI Yu-feng1,ZHAN Peng-fei2,FEN Jian-qing1,ZHU Xiangrui2* (1. Huzhou Agricultural Academy of Zhejiang province,Huzhou 313000,China,2. College of Animal Sciences,Zhejiang University,Hangzhou 310029,China) |
| |
| Abstract: | Selected the extract conditions of ramulus mori polysaccharide by orthogonal design, and combination of microwave extraction,ultrasonic extraction and enzymatic extraction method the effect of extraction was observed. we compared the ethanol precipitation method and the macroreticular resin purification effect by purify the crude extract. The results were that the best extract conditions were the time being 100min, the solution-material ratio being more than 20 times and pH5 in general extraction method. Th... |
| |
| Keywords: | Mulberry branch Polysaccharide Extraction Purification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
