| 菊苣农杆菌介导转化受体系统的研究(简报) |
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| 引用本文： | 程林梅,孙毅,王亦学,吴家和.菊苣农杆菌介导转化受体系统的研究(简报)[J].草业学报,2008,17(1):130-134. |
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| 作者姓名： | 程林梅 孙毅 王亦学 吴家和 |
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| 作者单位： | 山西省农业生物技术研究中心,山西,太原,030031;山西省农业科学院棉花研究所,山西,运城,044000 |
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| 基金项目： | 山西省自然科学基金
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山西省农业科学院高新项目 |
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| 摘 要： | 对影响菊苣转化受体系统几种重要因素进行了初步探讨。研究结果表明,采用菊苣叶片、茎段作为外植体,在MS 6-BA 1.5 mg/L IBA 0.2 mg/L培养基上,再生频率达100%,农杆菌菌株LBA4404介导菊苣遗传转化体系中,经生根后20~25 d的叶片,遗传转化效果最佳。共培养2 d,同时附加20~25 mg/L卡那霉素和500~600mg/L头孢霉素,转化频率由1.5%提高到7.1%左右。
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| 关 键 词： | 菊苣 农杆菌介导 受体系统 转化 |
| 文章编号： | 1004-5759（2008）01-0130-05 |
| 收稿时间： | 2007-04-17 |
| 修稿时间： | 2007-08-14 |
Research on a transformation acceptor system of Cichoriun intybus mediated by Agrobacterium tumefaciens |
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| CHENG Lin-mei,SUN Yi,WANG Yi-xue,WU Jia-he.Research on a transformation acceptor system of Cichoriun intybus mediated by Agrobacterium tumefaciens[J].Acta Prataculturae Sinica,2008,17(1):130-134. |
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| Authors: | CHENG Lin-mei SUN Yi WANG Yi-xue WU Jia-he |
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| Abstract: | Several important influential factors on a transformation acceptor system of Cichoriun intybus were studied and it was shown that the transformation frequency was 100%,when the leaves and caudexes as explants were cultivated on a co-culture medium of MS 6-BA 1.5 mg/L IBA 0.2 mg/L.Furthermore,the genetic transformation of leaves for 20-25 d after rhizogenesis was most effective using the genetic transformation system of C.intybus mediated by Agrobocterium tumefaciens LBA4404.When the C.intybus leaves were co-cultured for 2 d with added ranamycim(20-25 mg/L) and cefotaxime(500-600 mg/L),the transformation frequency increased from 1.5% to about 7.1%. |
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| Keywords: | Cichoriun intybus mediation by Agrobacterium tumefaciens acceptor system transformation |
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