柳枝稷人工穗芽高效再生体系的建立

以柳枝稷‘西稷1号’、‘西稷2号’和‘西稷3号’3个基因型的幼穗为材料,采用单因素试验依次从基因型、幼穗长度、激素、初次切割时期穗芽长度、大量培养阶段穗芽长度、碳源、pH值、穗芽块的切割方式和生根培养基等对柳枝稷人工穗芽再生体系进行了优化研究,建立了柳枝稷人工穗芽高效再生体系,即基因型选用西稷1号,幼穗长度为1.5 cm,激素采用3.0 mg/L 6-BA+0.1 mg/L 2,4-D, 初次切割时期和大量培养阶段穗芽长度均为1.5 cm,碳源采用麦芽糖,pH值5.8,穗芽块采用纵切方式,并以1/2 MS培养基作为生根培养基,增殖效率可高达4 167.00%。

Establishment of a highly efficient regeneration system of switchgrass artificial spike buds

With inflorescence of three genotypes of Xiji No.1,Xiji No.2,Xiji No.3 as materials,using single factor design consider factors including genotype,panicle length,phytohormone,the length of spike buds in the initial cutting period and in mass cultivating period,the carbon source,pH,spike buds cutting way and root induction medium,to optimize the regeneration system of switchgrass(Panicum virgatum) artificial spike buds.The optimal regeneration system was: genotype Xiji No.1,panicle length 1.5 cm,3.0 mg/L 6-BA+0.1 mg/L 2,4-D as phytohormone,the length of spike buds in the initial cutting period and in mass cultivating period was 1.5 cm,maltose as carbon source,pH 5.8,vertical spike buds cutting,and 1/2 MS as root induction medium.The proliferation efficiency of switchgrass artificial spike buds might reach up to 4 167.00%.