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苜蓿种带真菌及其致病性测定
引用本文:李春杰,南志标.苜蓿种带真菌及其致病性测定[J].草业学报,2000,9(1):27-36.
作者姓名:李春杰  南志标
作者单位:甘肃草原生态研究所,甘肃兰州 730020
基金项目:农业部“九五”畜牧业重点项目专题!(95牧-02-07-01)“我国主要豆科牧草重大病害及防治技术研究”的部分内容
摘    要:对来自我国新疆,内蒙古,吉林,陕西,甘肃,宁夏,山东,黑龙江,山西,河北和江苏等11个省(自治区)苜蓿产区的30个审定登记品种的38个苜蓿种子样品进行了系统的种带真菌检测研究;测定了21种25个真菌分离物对苜蓿幼苗根组织的入侵性及19种真菌对苜蓿种子萌发和幼苗生长的影响。

关 键 词:苜蓿  种带真菌  致病性测定  致病菌
文章编号:1004-5759(2000)01-0027-10
修稿时间:1999年6月22日

Seed-borne fungi of lucerne and their pathogenicity to lucerne seed and seedling
LI Chun jie,NAN Zhi biao.Seed-borne fungi of lucerne and their pathogenicity to lucerne seed and seedling[J].Acta Prataculturae Sinica,2000,9(1):27-36.
Authors:LI Chun jie  NAN Zhi biao
Abstract:Thirty eight seed samples belonging to 30 registered lucerne varieties were collected and tested for seed borne fungi. The seed samples were produced in 11 provinces(Automonous Regions) in China, which included Xinjiang, Inner Mongolia, Jilin, Shaanxi, Gansu, Ningxia, Shandong, Heilongjiang, Shanxi, Hebei and Jiangsu. Invasiveness of 25 isolates of 21 fungal species from seed to seedling root tissue and effects of 19 fungal species on seed germination and seedling growth of lucerne were tested. Percentage frequency of fungi isolated from each seed sample was 0.75% to 100%, and 40 fungal species of 36 genera were identified. Among them, Penicillium spp., Alternaria alternata (Fr.) Keiss., Trichothecium roseum(Bull.) Link, Aspergillus niger Tiegh., Cladosporium herbarum(Pers.) Link and A.flavus Link were isolated most frequently and caused damage to seed and seeling in some extent in invasiveness test. The pathogenicity tests showed that Fusarium oxysporum Schlecht., F.avenaceum (Fr.) Sacc., Ascochyta imperfecta Peck, Rhizoctonia solani Kuhn, Bipolaris sorokiniana (Sacc. et Sorok..) Shoem, Diplodia sp., Alt. alternata and Cl.herbarum were the seed borne pathogeic fungi of lucerne. Compared to uninoculated control, all 19 fungal species tested reduced lucerne seed germination and increased root rot index significantly (P<0.05), whereas F.oxysporum killed all seeds tested.
Keywords:lucerne  seed  borne fungi  pathogenicity test  seed  borne pathogenic fungi
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