箭筈豌豆种子人工加速老化条件筛选的研究

人工加速老化法是种子活力测定的常用方法之一,但不同物种适宜的老化条件往往不同。以9个箭筈豌豆种批为材料,在100%相对湿度,41、43及45 ℃条件分别老化处理12、24、36、48、60及72 h,通过测定发芽率及发芽速率,筛选出箭筈豌豆适宜的人工加速老化条件。结果表明,老化温度、时间以及两者的交互作用对发芽率及发芽速率均有极显著影响(P<0.01)。随着老化温度及时间的增加,发芽率逐渐下降,老化处理48 h后发芽率均显著降低(P<0.05)(除种批6);发芽速率先增加后降低,24 h后呈现下降趋势。相关分析结果表明老化时间对箭筈豌豆发芽率和发芽速率的影响大于温度。在41 ℃-60 h老化条件下,发芽率和发芽速率分别将9个种批分为8和6个质量等级,比其他老化处理条件划分更多的质量等级,且各质量等级之间差异显著(P<0.05)。因此,41 ℃-60 h是箭筈豌豆适宜的人工加速老化条件。

Optimized accelerated aging test for common vetch

Accelerated aging is a common method for seed vigor testing,but the outcome differs with different species.In this study nine seed lots of common vetch (Vicia sativa) were assessed;seeds were aged at 100％ relative humidity at 41,43 and 45 ℃ for 12,24,36,48,60 and 72 h,respectively.An optimal accelerated aging condition was selected by analyzing germination ％ and rate of germination.Results indicated that aging temperature,time and their interaction showed significant differences for germination ％ and germination rate (P＜0.01).With increasing temperature and time germination ％ gradually declined.Germination ％ decreased significantly after aging for 48 h (P＜0.05) (except for seed lot 6).The germination rate increased firstly and then decreased after 24 h.Correlation analysis showed that the effects of aging time on germination ％ and germination rate were greater than that of temperature.Aging seed for at 41 ℃ for 60 hours was able to separate the seed lots into groups of 8 or 6 based on germination ％ and germination rate,respectively (P＜0.05).We concluded that aging at 41 ℃ for 60 h was the optimal condition for accelerated age testing of common vetch.