扁茎黄芪离体快繁及多倍体诱导

以扁茎黄芪的干燥成熟种子为外植体,研究其离体快繁技术,并在无菌繁殖条件下探索了秋水仙碱溶液不同浓度和时间处理对扁茎黄芪的诱变效应。快繁研究结果显示,扁茎黄芪的发芽培养基为MS,继代增殖最适培养基为MS+6-BA3.0mg/L+NAA0.5mg/L,平均增殖系数达到4.5,平均苗高达3.5cm;生根最适培养基为1/2MS+NAA0.05mg/L,生根数为4～6条,生根率达到100%。采用浸泡法将扁茎黄芪的茎尖在0.1%,0.3%和0.5%不同秋水仙碱溶液浓度下分别处理1,2,3和4d,并对诱导处理后获得的再生植株进行染色体鉴定,得出以0.3%秋水仙碱溶液浸泡3d为诱导多倍体的最佳处理组合,诱导率达40%。

Rapid propagation in vitro and polyploid induction of Astragalus complanatus

Dry and ripe seeds of Astragalus complanatus were used as explants for rapid propagation to compare the mutagenic effects of different concentrations and durations of colchicine on A.complanatus.Rapid propagation experiments showed that MS was the optimal medium for buds,while MS+6-BA 3.0 mg/L+NAA 0.5 mg/L was the optimal proliferation medium with an average proliferation coefficient of 4.5 and average plantlet height of 3.5 cm.The best rooting medium was 1/2 MS+NAA 0.05 mg/L with a root count of 4-6 and 10...