| 柱花草愈伤组织培养与植株再生(简报) |
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| 引用本文： | 刘进平,吴发红,王丹,刘杨晶,朱涛.柱花草愈伤组织培养与植株再生(简报)[J].草业学报,2007,16(3):136-138. |
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| 作者姓名： | 刘进平 吴发红 王丹 刘杨晶 朱涛 |
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| 作者单位： | 华南热带农业大学农学院,海南,儋州,571737 |
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| 摘 要： | 对热研2号柱花草无菌实生苗的胚轴与叶片切段进行愈伤组织培养研究。结果表明,MS NAA 2 mg/L BA 0.5 mg/L及MS 2,4-D 3 mg/L BA 0.3~0.5 mg/L CH 200 mg/L对叶片切段诱导效果最好。而下胚轴愈伤组织的诱导以MS NAA 3 mg/L BA 0.1~0.3 mg/L和MS 2,4-D 3 mg/L BA 0.5 mg/L最优。愈伤组织在MS BA 2.5 mg/L NAA 0.1 mg/L进行分化培养,可实现高频植株再生。最适生根培养基为1/2MS 1.0mg/L IBA 0.5 mg/L IAA,生根率可达95.84%。
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| 关 键 词： | 热研2号柱花草 组织培养 愈伤组织 植株再生 |
| 文章编号： | 1004-5759（2007）03-0136-03 |
| 收稿时间： | 2006-04-25 |
| 修稿时间： | 2006-04-25 |
Callus culture and plant regeneration of Stylosanthes guianensis |
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| LIU Jin-ping,WU Fa-hong,WANG Dan,LIU Yang-jing,ZHU Tao.Callus culture and plant regeneration of Stylosanthes guianensis[J].Acta Prataculturae Sinica,2007,16(3):136-138. |
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| Authors: | LIU Jin-ping WU Fa-hong WANG Dan LIU Yang-jing ZHU Tao |
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| Institution: | Agronomy College, South China University of Tropical Agriculture, Danzhou 571737, China |
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| Abstract: | Studies on callus culture and plant regeneration of Stylosanthes guianensis cv.Reyan 2 showed that the optimum callus induction media were MS NAA 2 mg/L BA 0.5 mg/L and MS 2,4-D 3 mg/L BA 0.3-0.5 mg/L CH 200 mg/L for leaf pieces,and MS NAA 3 mg/L BA 0.1-0.3 mg/L and MS 2,4-D 3 mg/L BA 0.5 mg/L for hypocotyl segments from aseptic seedlings.High-frequency bud differentiation and plant regeneration was achieved on MS BA 2.5 mg/L NAA 0.1 mg/L.The best rooting medium was 1/2 MS 1.0 mg/L IBA 0.5 mg/L IAA. |
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| Keywords: | Stylosanthes guianensis cv Reyan2 tissue culture callus plant regeneration |
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