雄性食蟹猴生殖系统中Ghrelin的分布定位 |
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作者姓名: | 李鑫 宋尚桥 马围围 郑福 曾素先 孙翠翠 严瑾 黎宗强 |
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作者单位: | 广西大学动物科学技术学院;南宁市江南区沙井街道办事处水产畜牧兽医站 |
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基金项目: | 广西壮族自治区科技计划项目-重点研发计划(桂科AB16380072)。 |
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摘 要: | 本文采用免疫组织化学染色方法,探讨ghrelin在雄性食蟹猴生殖系统内的分布定位。通过对ghrelin免疫阳性细胞在生殖系统中分布部位、含量及细胞形态等方面的研究,为今后ghrelin在食蟹猴体内的功能研究奠定形态学基础。免疫组化染色发现,ghrelin阳性细胞在食蟹猴的生殖系统中有分布。Ghrelin免疫阳性细胞被染为棕色到棕黑色,主要分布于睾丸、附睾及输精管中,精囊腺中无ghrelin阳性细胞分布。Ghrelin阳性细胞在组织中多呈散在分布。细胞大小不一、形态各异,多呈圆形、卵圆形、锥体形、长柱形及其他不规则形。
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关 键 词: | GHRELIN 食蟹猴 生殖系统 免疫组化定位 |
Study on Immunohistochemical Localization of Ghrelin in the Reproductive System of Male Macaca fascicularis |
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Authors: | LI Xin SONG Shangqiao MA Weiwei ZHENG Fu ZENG Suxian SUN Cuicui YAN Jin LI Zongqiang |
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Institution: | (College of Animal Science and Technology,Guangxi University,Nanning,530005,China;Shajing Subdistrict Office,Jiangnan District,Nanning City Aquatic Animal Husbandry and Veterinary Station,Nanning,530033,China) |
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Abstract: | We used immunohistochemistry to investigate the localization of ghrelin in the reproductive system of Macaca fascicularis.Immunohistochemical staining showed that ghrelin-positive cells were recorded in the reproductive system of M.fascicularis.Here,ghrelin-immunoreactive cells were stained brown to brown-black,and were mainly distributed in the testis,epididymis and spermaduct,but not in the seminal vesicle.Ghrelin cell distribution was scattered in tissues.The sizes and shapes of cells varied but mainly were round,oval,spindle-shaped,columnar,and of other irregular shapes. |
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Keywords: | Ghrelin Macaca fuscicularis Reproductive system Immunohistochemical localization |
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