家蚕绵茧突变性状调查

家蚕绵茧(Flossy)突变系二化性、单基因显性突变,表型为茧形浮松、多分层,是几个较为重要的蚕茧突变之一。本文选取夏芳、大造作为对照,对绵茧的茧层率、含胶率、茧丝显微镜观察、丝胶蛋白SDS-PAGE进行调查比较。实验结果显示,夏芳、大造、绵茧茧层率为23.476%、12.919%、15.551%;含胶率依次为24.980%、33.765%、28.399%;夏芳、大造、绵茧茧层显微镜观察结果未显示明显差异;丝胶蛋白成分分析结果显示,绵茧和对照之间没有明显不同。就目前结果来看,丝胶蛋白含量有可能是致使绵茧表型的原因,但与对照的差异不明显,因而绵茧突变性状形成的真正原因有待进一步研究。本研究为家蚕绵茧基因的克隆及功能研究提供参考信息。

Investigation of the Mutant Character Flossy in the Silkworm,Bombyx mori

ilkworm is one of the most important model insects.Further more,it is an important genetic material.Currently,there are more than 400 silkworm mutants preserved in Silkworm Resource Library.Research of the underlying physiological and biochemical mechanisms for the generation of silkworm mutant phenotypes can provide effective clews for positional cloning research.Flossy mutant is a digoneutism,single gene dominant mutant,whose phenotype is characterized by floating loose cocoons with much layering,and Flossy is one of the outbalance silkworm cocoon mutants.In this study,Xiafang and Dazao were chosen as the control,the cocoon shell rate and the sericin holding-rate of Flossy were observed microscopically and its sericin protein was analyzed with SDS-PAGE.The results showed that the cocoon shell rate of Xiafang,Dazao and Flossy was 23.476%,12.919% and 15.551%;and their the sericin holding-rate was 24.980%,33.765% and 28.399%,respectively.Microscopic examination revealed no outstanding difference in cocoon shell among Xiafang,Dazao and Flossy.The analysis of sericin proteins also showed little difference between Flossy and the control.In conclusion,the level of sericins proteins might be the reason for the Flossy phenotype,but the difference between the mutant and the control in this respect was non-significant.Therefore,more research is needed to clarify the exact mechanism for the generation of the Flossy mutant character.