荧光定量PCR技术在疾病检测上的应用

实时荧光定量PCR技术融汇了传统PCR技术灵敏、快速、特异的特点以及光谱技术的高敏感性和高精确定量的优点,以直接探测PCR过程中荧光信号的变化获得定量的结果。因其具有特异性强、灵敏度高、重复性好、定量准确、速度快、全封闭反应等优点,成为当前疫病检测广泛采用的分子生物学诊断技术。本文就实时荧光定量PCR技术在疾病检测上的应用作一综述。

Application of Real-time Fluorescent Quantitative PCR on Disease Detection

The real-time fluorescent quantitative PCR technology combines the traditional PCR and spectroscopy's advantages.It is more sensitive,rapid and specific.It can detect the changes of fluorescencesignal in the process of PCR to obtain quantitative results.It's molecular biology diagnostic techniques widely used in disease detections because of it's specificity,sensitivity,reproducibility,quantity,rapid and capping.In this paper,real-time fluorescent quantitative PCR technology or diagnosis of diseases was rev...