以超滤技术浓缩猪细小病毒含毒细胞液的试验

在研制猪细小病毒灭活疫苗时，应用了超滤浓缩技术，提高了合毒细胞培养液中的抗原含量。为了保证浓缩的流量，减少对滤膜的污染，对含毒细胞培养液作了预处理一离心及微滤，以便除去细胞培养液中的细胞碎片、蛋白质等大分子物质及固形微粒。超滤时选用50000Da滤膜，在20psig压力下，经过5倍浓缩的PPV细胞培养液，血凝滴度提高2个滴度以上。病毒含量测定表明，TCID50／0．2mL由107左右升至109以上，以其配制疫苗，能显著地提高疫苗的免疫原性。超滤技术具有易于操作、高效、分离精度高、没有二次污染等优点，可以根据需要选择不同的浓缩浓度，对保证疫苗的质量具有重要作用。

The Test for Concentrating the Cell Culture Medium of Pig Parvovirus with Ultrafiltration Technology

The antigen content were improved by uhrafihration concentration technology in toxic cell culture medium, when the inactivated vaccine for pig parvovirus were developed.Ensuring the concentrated flow and reducing the filtration membrane pollution, the toxic cell culture medium were pretreated by centrifugal and mi- cro-filtration, in order to eliminate macromolecular material for cell debris, protein in cell culture medium and solid particles. The titers of hemagglutination were increased more than 2 under the condition of 50000Da filtration membrane, in 20 psig pressure,using five times concentrated PPV cell culture medium in Uhrafihration. The virus content determination showed that TCIDs0/0.2mL from about 107to 109above, the vaccine immunogenicity were significantly improved with its preparation vaccine. The ultrafiltration technology has the advantages of easy opera- tion, high efficiency, high separation accuracy and no secondary pollution etc, It can play an important role for the quality of vaccine according to the different needs of the enrichment concentration.