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奶牛粪中致病性大肠杆菌的分离鉴定及生物学特性的研究
引用本文:于俊娥,敖日格乐,王纯洁,张爱荣,格日勒图,程佳.奶牛粪中致病性大肠杆菌的分离鉴定及生物学特性的研究[J].畜牧兽医杂志,2007,26(5):1-3.
作者姓名:于俊娥  敖日格乐  王纯洁  张爱荣  格日勒图  程佳
作者单位:内蒙古农业大学动物科学与医学学院,呼和浩特,010018
摘    要:对呼和浩特市地区不同奶牛场共20头健康荷斯坦奶牛的鲜粪便进行了病原性大肠杆菌的分离提取,共分离到大肠杆菌40株,经鉴定病原性大肠杆菌6株,均为强毒株,占分离菌数的15.0%,而34株为非致病性大肠杆菌,占分离菌数的85.0%。同时对6株病原性大肠杆菌进行了病原性特性的研究,发现病原性菌的生化特性基本一致,与资料报道相符。对6株病原性大肠杆菌进行了抗"O"血清鉴定,除1株有自凝现象,其余5株分属O1、O2、O83个血清型,分别占定型菌株的20%、40%和40%。

关 键 词:病原性大肠杆菌  生物学特性  鉴定  奶牛粪便
文章编号:1004-6704(2007)05-0001-03
修稿时间:2006-12-14

Isolation and Identification of Pathogenic Escherichia coli from Cow Dung and Study of its Biological Characteristics
YU Jun-e,AO Rige-le,WANG Chun-jie,ZHANG Ai-rong,GE Rile-tu,CHENG Jia.Isolation and Identification of Pathogenic Escherichia coli from Cow Dung and Study of its Biological Characteristics[J].Journal of Animal Science and Veterinary Medicine,2007,26(5):1-3.
Authors:YU Jun-e  AO Rige-le  WANG Chun-jie  ZHANG Ai-rong  GE Rile-tu  CHENG Jia
Institution:Animal Science and Medicine, Inner Mongolia Agricultura University, Huhhot 010018 ,China
Abstract:40 strains of Escherichia coli were isolated from 20 healthy Holland stan cow's fresh feces in different districts in Huhhot and identicated as pathogenic Escherichia coli.Results showed that 6 strains among them were high pathogenicity(15.0%),34 strains were non-pathogenicity(85.0%).Biochemical test result of 6 strains high pathogenic Escherichia coli was nearly the same.The results of serotyping showed that 5 strains belong to 3 serotypes: O1,O2,O8,and account for 20%,40%,40% respectively,in the pathogenic Escherichia coli except 1 strain which could not be serotyped.
Keywords:pathogenic Escherichia coli  biological characteristics  identification  cow dung
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