| 藏獒RAPD-PCR反应体系的优化 |
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| 引用本文： | 兰小平,李三相,李一婧,王廷璞,黄晶,鄢珣,崔泰保.藏獒RAPD-PCR反应体系的优化[J].畜牧兽医杂志,2010,29(1):1-3. |
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| 作者姓名： | 兰小平 李三相 李一婧 王廷璞 黄晶 鄢珣 崔泰保 |
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| 作者单位： | 1. 天水师范学院生命科学与化学学院,甘肃,天水,741000
2. 甘肃农业大学 |
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| 摘 要： | 以藏獒血液为试验材料,提取基因组DNA后,对藏獒RAPD反应体系进行了优化。结果表明,20μL的最佳反应体系为:2.5 mmol/LMg2+、0.6 mmol/L dNTP、50 ng模板DNA、1.25UTaq酶和0.3μmol/L引物;藏獒RAPD反应的最佳退火温度为36.5℃。
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| 关 键 词： | 藏獒 RAPD—PCR 体系优化 |
Optimization of RAPD-PCR Reaction System for Tibetan Mastiff |
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| LAN Xiao-ping,Li San-xiang,LI Yi-jing,WANG Ting-pu,HUANG Jing,YAN Xun,CUI Tai-bao.Optimization of RAPD-PCR Reaction System for Tibetan Mastiff[J].Journal of Animal Science and Veterinary Medicine,2010,29(1):1-3. |
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| Authors: | LAN Xiao-ping Li San-xiang LI Yi-jing WANG Ting-pu HUANG Jing YAN Xun CUI Tai-bao |
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| Institution: | 1. School of Life science and Chemistory, Tianshui Normal University , Tianshui 741000, China ; 2. Cansu Agricultural University) |
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| Abstract: | The RAPD -PCR reaction system was optimized after the genomic DNA was extracted from the blood of Tibetan Mastiff. The results showed that a total volume of 20 μL optimal RAPD - PCR reaction system of Tibetan Mastiff consisted of 2.5 mmol/L Mg2＋ ,0.6mmol/LdNTP,50ng genomic DNA, 1.25U Taq DNA polymerase , and 0.3 μmol;L primers. The best renaturation temperature of RAPD analysis of Tibetan Mastiff was 36.5℃. |
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| Keywords: | RAPD-PCR |
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