疆岳驴催乳素受体基因多态性及其与泌乳性状间的相关性分析

试验旨在研究疆岳驴催乳素受体（prolactin receptor，PRLR）基因多态性及其与泌乳性状间的关系，探寻可用于疆岳驴选育的分子遗传标记。随机选取120头疆岳驴，对疆岳驴的泌乳性能指标进行测定，并运用PCR-SSCP方法对疆岳驴PRLR基因侧翼区进行多态性分析。结果显示，PRLR基因在疆岳驴群体中存在2种基因型：AA和AB，A为优势等位基因；测序结果显示，PRLR基因的碱基突变位置为g.29764584 C>G，该突变为同义突变，没有导致编码的氨基酸发生改变；经关联分析，PRLR基因AB基因型个体平均日泌乳量显著高于AA基因型（P<0.05），AA基因型个体乳蛋白率显著高于AB基因型（P<0.05），在乳糖率上差异不显著（P>0.05）。疆岳驴PRLR基因突变与泌乳性状存在关联性，推测PRLR基因可以作为疆岳驴乳用型选育的分子遗传标记之一。

Study on the Relationship between the Polymorphisms of PRLR Gene and Lactation Traits in Jiangyue Donkeys

In this study, the single nucleotide polymorphism of prolactin receptor (PRLR) gene and its correlation with the lactation traits in Jiangyue donkeys was tested, and the molecular genetic marker of breeding Jiangyue donkeys was explored.120 individuals were analyzed association of PRLR gene polymorphism with lactation traits in Jiangyue donkeys. Genetic polymorphism of PRLR gene flanking region was detected by PCR-SSCP method. The results showed that there was 1 mutation in PRLR gene and 2 genotypes of AA and AB, and allele A was the dominant allele. The SNP g.29764584 C>G was identified of PRLR gene by sequencing, and it was a synonymous mutation, did not cause amino acid changes. Statistical results indicated that the SNP was significantly associated with average daily milk yield and protein percentage in Jiangyue donkey (P<0.05),there was no significant in the lactin percentage(P>0.05). The mutation might be as crucial DNA genetic marker for lactation performance selection in Jiangyue donkeys.