Gene Analysis of Penton Protein of 12 Serotypes of Fowl Adenovirus

In order to understand the genetic evolution of Penton protein of fowl adenovirus(FAdV),the specific primers of Penton were designed on the basis of published sequences of different genotypes on GenBank.Then Penton gene of 12 serotypes were amplified by PCR and constructed into pEASY-Blunt Simple Cloning Vector for sequencing.The nucleotide sequences of Penton protein of 12 serotypes were analyzed and compared with the nucleotide sequences of standard strains published on NCBI by ClustalW method to draw a genetic phylogenetic tree.The results showed that the nucleotide sequences of Penton protein of 12 serotypes had more than 99.2% homology with their respective standard strain,among which the homology of FAdV-1,FAdV-2,FAdV-3,FAdV-4,FAdV-6,FAdV-7,FAdV-8a,FAdV-8b,FAdV-10 and FAdV-11 were as high as 100%.This further confirmed the consistency between the laboratory preserved strains and the world standard strains.However,although Penton protein was highly conserved,there were still significant differences among different species.The nucleotide homology between FAdV-A1 and other species was 70.6%-73.3%,while that of FAdV-B5 was 70.6%-77.9%.The nucleotide homology between FAdV-C strains of different serotypes was 99.6%,while homology between FAdV-C4 and other species was 71.2%-73.4%.The nucleotide homology between FAdV-D strains of different serotypes was 95.6%-98.7%,while the highest homology between FAdV-D and other species was 85.3%.The nucleotide homology between FAdV-E strains of different serotypes was 98.2%-99.4%,while the highest homology between FAdV-E and other species was 85.3%.In conclusion,the homology difference between strains of the same species was small,and that of different species was significant.FAdV could be divided into A,B,C,D and E species according to nucleotide sequence of Penton protein.This study successfully cloned 12 serotypes Penton gene of FAdV and performed genetic evolution analysis,which laid the foundation for the diagnosis,monitoring and identification of FAdV in the future.