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| 马绒毛膜促性腺激素单克隆抗体的制备及鉴定 | |
| 引用本文： | 苏晓倩,邓亮,贾瑜,韩国才.马绒毛膜促性腺激素单克隆抗体的制备及鉴定[J].中国畜牧兽医,2012,39(9):18-21. |
| 作者姓名： | 苏晓倩 邓亮 贾瑜 韩国才 |
| 作者单位： | 1. 中国农业大学动物科技学院,北京 100193; 2. 北京勤邦生物技术有限公司,北京 100026; 3. 中国农业大学马研究中心,北京 100193 |
| 基金项目： | 国家公益性行业(农业)科研专项 |
| 摘 要： | 本研究采用马绒毛膜促性腺激素(equine chorionic gonadotropin,eCG)纯品免疫BALB/c小鼠,取其脾细胞与同系小鼠骨髓瘤细胞SP2/0按1∶10融合,间接酶联免疫吸附法(ELISA)筛选阳性克隆和有限稀释法克隆,扩大培养后于小鼠腹腔制备腹水,共得到8株能稳定分泌单克隆抗体的杂交瘤细胞株,采用间接ELISA测定抗体效价,效价均达10-5以上。用获得的杂交瘤细胞株制备单克隆抗体,为eCG分泌水平的ELISA检测方法的建立确立了条件。 |
| 关 键 词： | 马绒毛膜促性腺激素 杂交瘤细胞株 单克隆抗体 酶联免疫吸附法 |
| 收稿时间： | 2012-02-27 |
Preparation and Identification of Monoclonal Antibody Against Equine Chorionic Gonadotropin |
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| SU Xiao-qian , DENG Liang , JIA Yu , HAN Guo-cai.Preparation and Identification of Monoclonal Antibody Against Equine Chorionic Gonadotropin[J].China Animal Husbandry & Veterinary Medicine,2012,39(9):18-21. | |
| Authors: | SU Xiao-qian DENG Liang JIA Yu HAN Guo-cai |
| Institution: | 1. College of Animal Science and Technolgy, China Agricultural Univeristy, Beijing 100193, China; 2. Beijing Qinbang Biological Technology Co., Ltd.,Beijing 100026, China; 3. Equine Research Center, China Agricultural Univeristy, Beijing 100193, China |
| Abstract: | This study fusing the spleen cells of BALB/c mice which was immunized with high purified equine chorionic gonadotropin and myeloma cells SP2/0 at the rate of 1∶10,and screening the positive cloning by the means of indirect enzyme linked immunosorbent assay.Positive cells were clone through limited dilution method,injecting the mixture into the abdominal cavity of mice after intermediate cultured.Antibody titer was assayed by ways of indirect ELISA.Eight hybridoma cell lines were obtained which could secrete eCG stably,titer reached above 10-5.The obtained eCG hybridoma cell lines,which laid a foundation to assay the level of eCG. |
| Keywords: | equine chorionic gonadotropin hybridoma cell line monoclonal antibody enzyme linked immunesorbent assay |
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