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3株柔嫩艾美耳球虫28SrRNA基因部分序列的扩增与分析
引用本文:侯杰,林瑞庆,刘丽丹,陈俊嘉,翁亚彪.3株柔嫩艾美耳球虫28SrRNA基因部分序列的扩增与分析[J].中国畜牧兽医,2012,39(7):43-46.
作者姓名:侯杰  林瑞庆  刘丽丹  陈俊嘉  翁亚彪
作者单位:1. 华南农业大学兽医学院,广东广州,510642
2. 佛山市正典生物技术有限公司,广东佛山,528138
基金项目:广东省自然科学基金,广东省科技计划项目
摘    要:本研究应用PCR技术扩增来自广东的3株柔嫩艾美耳球虫的28S rRNA基因部分序列,并与GenBankTM登录的柔嫩艾美耳球虫、堆型艾美耳球虫、鼠肉孢子虫和刚地弓形虫虫株的相应序列进行比对分析。试验结果显示,柔嫩艾美耳球虫3个样品均获得1172 bp的28S rRNA基因部分有效序列,不同虫株序列没有差异,与GenBankTM登录的柔嫩艾美耳球虫相应序列只有一个碱基差异,显示种内序列高度保守,而与堆型艾美耳球虫、鼠肉孢子虫、刚地弓形虫相应的序列存在不同程度的差异。结果表明,28S rRNA基因部分序列可作为研究艾美耳球虫种间及其他顶复门原虫遗传变异的标记。

关 键 词:柔嫩艾美耳球虫  28S  rRNA  PCR  序列分析  
收稿时间:2011-11-07

PCR Amplification and Sequence Analysis of Partial 28S rRNA Sequence of three Eimeria tellena Strains
HOU Jie , LIN Rui-qing , LIU Li-dan , CHEN Jun-jia , WENG Ya-biao.PCR Amplification and Sequence Analysis of Partial 28S rRNA Sequence of three Eimeria tellena Strains[J].China Animal Husbandry & Veterinary Medicine,2012,39(7):43-46.
Authors:HOU Jie  LIN Rui-qing  LIU Li-dan  CHEN Jun-jia  WENG Ya-biao
Institution:1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;2. Foshan Standard Bio-Tech Co. Ltd., Foshan 528138, China
Abstract:The partial 28S ribosomal RNA sequences of three different Eimeria tellena strains isolated from Guangdong province were amplified by polymerase chain reaction(PCR),and compared with relevant sequences of E.tellena,E.acervulina,Sarcocystis muris,T.gondii available in GenBankTM.The results showed that the partial 28S rRNA sequence of three E.tellena strains was 1172 bp in length with no nucleotide difference in each other,and only one base difference found with E.tellena in GenBankTM,revealing highly conservative within E.tellena.There were distinct sequence differences found comparing with E.acervulina,S.muris and T.gondii,which demonstrated 28S rRNA could be used as a genetic marker for the population genetic studies on different species of Eimeria and other apicomplexan parasites.
Keywords:Eimeria tellena  28S rRNA  PCR  sequence analysis
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