RT-PCR方法检测犬瘟热的流行情况

犬瘟热是由犬瘟热病毒引起的犬科烈性传染病,给患病动物带来重大危害。本研究基于犬瘟热病毒的NP蛋白基因设计特异性引物,利用RT-PCR获得287 bp目的片段,经TA克隆并测序。结果表明,该片段与NCBI上公布的犬瘟热病毒NP序列(登录号:EU716322)同源性达到98%。利用该特异性的RT-PCR方法,检测杭州市及周边部分地区犬瘟热的流行情况,结果表明,整个杭州及周边地区犬瘟热病毒总阳性检出率为6.7%,杭州城区阳性个体数最多,阳性率最高达到18.8%,其他地方(临安、台州、舟山)阳性率较低。该研究为犬瘟热的综合防制提供基础数据。

Epidemic of Canine Distemper Virus was Detected by RT-PCR

Canine distemper caused by canine distemper virus(CDV)is acute infection disease of canine,which caused severe losses.In this study,specific primers recognized CDV nucleoprotein were designed,the 287bp production was amplified by RT-PCR.This production was cloned by TA cloning and sequenced,the results showed that there was a high homogeneity of 98%in nucleotide with canine distemper virus NP sequence(accession number:EU716322)published in the GenBank.Epidemic of canine distemper in Hangzhou and other cities nearby was investigated by this specific RT-PCR.The results showed that total positive detection rate of CDV was 6.7%,the number of positive individuals in Hangzhou city was largest and positive detection rate was most up to 18.8%,positive detection of other cities(Linan,Taizhou,Zhoushan)were lower.The study is basic to prevention and treatment of canine distemper.