鸡传染性法氏囊高免卵黄IgY抗体不同提取方法的比较

本试验旨在提高鸡传染性法氏囊卵黄抗体防制鸡传染性法氏囊的效果,比较不同提纯方法对鸡传染性法氏囊高免卵黄抗体效价的影响。制备高免蛋并测定其效价,在实验室通过直接离心法、酸化水提取法、氯仿提取法、聚乙二醇法4种方法分别对高免卵黄抗体提取及抗体效价检测。试验结果表明,自制卵黄抗体效价为1:64;直接离心法的抗体效价为1:128,酸化水提取法的抗体效价为1:16,氯仿提取法抗体效价为1:256,聚乙二醇法的抗体效价是1:32。试验结果显示,使用氯仿提取法后检测的抗体效价最高,是临床提取抗体的常用方法。

Comparison of Different Extraction Methods of Chicken Infectious Bursal Disease the Yolk Antibody IgY

In order to improve prerention and control of infectious bursal IgY effect of infectious bursal,comparison of different purification method on the high IBD yolk antibody titer of effection. Preparation of hyper-immune eggs and determination of the titer,in the lab, four methods were used for extraction high-yolk antibodies and determination of the titer.They were direct centrifugation, acidfied water extraction, chloroform extraction method and polyethylene glycol method. The results showed that self-prepared yolk antibody titer was 1:64, direct centrifugation antibody titer was 1:128, acidfied water extraction antibody titer was 1:16, chloroform extraction method antibody titer was 1:256, polyethylene glycol method antibody titer was 1:32.The results showed that after using titer chloroform extraction method,the highest titer detected, chloroform extraction method was a common method of clinical extracted antibody.