| 日本乙型脑炎病毒小鼠脑内分布研究 |
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| 引用本文： | 马丽敏,李琳,张宁,胡乐鹏,李晔,何叶,任秀梅,李志萍,薛慧亮,李丹,靳朝,夏志平.日本乙型脑炎病毒小鼠脑内分布研究[J].中国畜牧兽医,2012,39(2):225-227. |
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| 作者姓名： | 马丽敏 李琳 张宁 胡乐鹏 李晔 何叶 任秀梅 李志萍 薛慧亮 李丹 靳朝 夏志平 |
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| 作者单位： | 1. 吉林大学畜牧兽医学院,吉林长春 130062;2. 中国人民解放军军事医学科学院军事兽医研究所,吉林长春 130122;3. 吉林农业大学,吉林长春 130017 |
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| 基金项目： | 国家高技术研究发展计划(863计划)课题(2009AA10Z4483730) |
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| 摘 要： | 为定位检测日本乙型脑炎病毒(JEV)在试验小鼠脑内分布情况,本研究建立了JEV的小鼠感染模型,取发病死亡小鼠的脑组织制作石蜡切片,利用抗JEV单克隆抗体,采用免疫酶组化方法对抗原进行组织学定位检测。试验结果表明,所建立的间接免疫酶组化方法灵敏度高,特异性好,证明了JEV选择性感染神经元,抗原阳性表达主要集中在脑干、神经节及大脑皮质。
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| 关 键 词： | 日本乙型脑炎病毒 间接免疫酶组化 抗原分布 |
Japanese Encephalitis Virus Distribution in the Brain of Mice |
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| MA Li-min,LI Lin,ZHANG Ning,HU Le-peng,LI Ye,HE Ye,REN Xiu-mei,LI Zhi-ping,XUE Hui-liang,LI Dan,JIN Zhao,XIA Zhi-ping.Japanese Encephalitis Virus Distribution in the Brain of Mice[J].China Animal Husbandry & Veterinary Medicine,2012,39(2):225-227. |
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| Authors: | MA Li-min LI Lin ZHANG Ning HU Le-peng LI Ye HE Ye REN Xiu-mei LI Zhi-ping XUE Hui-liang LI Dan JIN Zhao XIA Zhi-ping |
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| Institution: | 1. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2. Military Veterinary Institute,Academy of Military Medical Sciences of PLA,Changchun 130122,China;3. Jilin Agricultural Univesity,Changchun 130017,China |
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| Abstract: | To localize and detect the distribution of Japanese encephalitis virus(JEV) in the brain of experimental mouse,we established JEV infection model in mouse and produced paraffin sections using brain tissue.We established indirect immunohistochemical method with monoclonal antibodies of JEV to localize and detect the antigen.Experiment results showed that the method had a high sensitivity and specificity,it demonstrated that JEV infect neurons selectively,the antigen expression were mainly in the brainstem,ganglia and cortex. |
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| Keywords: | Japanese encephalitis virus indirect immunohistochemistry antigen distribution |
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