近期部分规模化猪场猪伪狂犬病野毒抗体监测情况调查

猪伪狂犬病仍然是我国猪群的重要威胁性传染病，通常造成母猪繁殖障碍以及仔猪的神经症状和高死亡率，同时伪狂犬病病毒也是猪呼吸系统疾病综合征的重要原发性病原。用gE-ELISA野毒鉴别诊断方法共检测了来自8个省、市46个猪场1940份血清样品，其中阳性样品657份，样品总阳性率33.87%，阳性猪场25个。在检测的各场中，阳性率最高猪场达到75.76%(125/165)。而部分进行了科学的疫苗免疫和实施了严格的生物安全措施的猪场始终保持猪伪狂犬病阴性(0/135)。利用基因缺失疫苗科学免疫配合伪狂犬抗体鉴别诊断技术是控制和净化伪狂犬病的重要手段。

Recent Epidemiological Surveillance of the Antibody to Field Pseudorabies Virus by Differential ELISA in Large-Scaled Swine Farms

Pseudorabies continued to spread throughout the swine farms in China and caused large economic loss for Chinese swine industry,It cause reproductive failure in breeding herds and neurological disorder and high mortality in piglet.pseudorabies virus(PRV) is also an important primary pathogen in porcine respiratory disease syndrome(PRDC).1940 serum samples from 46 large scaled swine farms located in 8 provinces were determined by differential PRV blocking gE-ELISA,it showed that 657(33.87%) samples were positive for antibody to gE of PRV,and 25 swine farms were positive for antibody to gE of PRV.Among these swine farms,the highest positive rate of field PRV infection is 75.76%(125/165).While some well-managed swine farms which performed scientific PRV immune procedure were negative for virulent field PRV infection.Gene deleted PRV vaccine(AUSKIPRA-GN) and commercial gE-ELISA reagent provide an ideal tool for the control and eradication of pseudorabies.