间接免疫细胞化学法快速检测新城疫病毒抗原的研究

本研究旨在建立一种简单、方便、准确、特异的方法检测新城疫病毒（Newcastle disease virus, NDV)。比较间接HRP-免疫细胞化学法与间接免疫荧光法两种方法检测NDV在COS-7细胞中的定位。两种试验方法都可以检测到NDV主要定位在COS-7细胞的胞浆内。间接HRP-免疫细胞化学法检测NDV要求一抗滴度≤1∶100，间接免疫荧光法要求的滴度≤1∶100。间接HRP-免疫细胞化学法检测NDV要求二抗滴度≤1∶300，间接免疫荧光法要求的滴度≤1∶50。而且结果观察中间接HRP-免疫细胞化学法要比间接免疫荧光法方便。间接HRP-免疫细胞化学法进行NDV检测优于间接免疫荧光法，是一种简单、快捷、准确的适用于检测NDV的技术。

Detection of NDV Infection and Antigen Location by Indirect Immunocytochemistry

The objective is to establish a simple, convenient, accurate, specific method to detect the NDV and detect the location of NDV in the COS-7 by comparing the indirect HRP-immunocytochemistry and indirect fluorescence immunocytochemistry. Both two experiment methods can detect the location of NDV and it was in the cell plasm of the COS-7. The indirect HRP-immunocytochemistry request the dilution of the first antibody is less than 1∶100, but the indirect fluorescence immunocytochemistry request the dilution of the first antibody is less than 1∶100. The indirect HRP-immunocytochemistry request the dilution of the second antibody is less than 1∶300, but the indirect fluorescence immunocytochemistry request the dilution of the second antibody is less than 1∶50. Further more, when detect the results that the indirect HRP-immunocytochemistry is more convenient than the indirect fluorescence immunocytochemistry. The indirect HRP-immunocytochemistry is simple, convenient, accurate and specific method to detect the NDV. 