首页 | 本学科首页   官方微博 | 高级检索  
     检索      

猫细小病毒CC-1株VP1基因克隆与序列分析
引用本文:李爽,YUAN Bao,肖书奇,REN Wen-zhi,张嘉保,ZHAO Zhi-hui,雍海平.猫细小病毒CC-1株VP1基因克隆与序列分析[J].中国畜牧兽医,2008,35(8).
作者姓名:李爽  YUAN Bao  肖书奇  REN Wen-zhi  张嘉保  ZHAO Zhi-hui  雍海平
作者单位:1. 吉林大学实验动物中心,长春130062;吉林大学畜牧兽医学院,长春,130062
2. 中山大学生命科学学院,广州,510275
3. 吉林大学实验动物中心,长春,130062
摘    要:根据GenBank登录的猫细小病毒(CU-4)VP1基因序列,设计了1对引物,用PCR技术对VP1基因进行整体扩增,将扩增产物纯化后克隆入PGM-T载体,通过酶切、PCR和测序进行验证。结果表明,测序拼接得出VP1基因的序列长度约为2306 bp,该基因的ORF总长为2184 bp,编码727个氨基酸,应用DNAStar软件把所测得的序列与国内外代表性毒株的VP1基因编码区全长核苷酸序列和氨基酸序列进行比对分析,发现CC-1株与国内XJ-1株的核苷酸和氨基酸同源性均为99.3%。

关 键 词:猫细小病毒  VP1基因  序列分析

Cloning and Sequencing of VP1 Gene of Feline Parvovirus CC-1 Strain
LI Shuang,YUAN Bao,XIAO Shu-qi,REN Wen-zhi,ZHANG Jia-bao,ZHAO Zhi-hui,YONG Hai-ping.Cloning and Sequencing of VP1 Gene of Feline Parvovirus CC-1 Strain[J].China Animal Husbandry & Veterinary Medicine,2008,35(8).
Authors:LI Shuang  YUAN Bao  XIAO Shu-qi  REN Wen-zhi  ZHANG Jia-bao  ZHAO Zhi-hui  YONG Hai-ping
Abstract:To analyze the sequence of VP1 gene of Feline parvovirus CC-1 strain and compare with that of representative virus strain.According to the reported complete nucleotide sequence of FPV(CU-4) in GenBank,a pair of primers were designed and synthesized.VP1 gene of isolate was amplified.The products of PCR were cloned into PGM-T vector.the positive clone was identified bu enzyme cutting,PCR and sequencing.The whole sequence of VP1gene was analyzed using DNAStar software.The results indicated that the VP1 gene with 2306 bp long had a single opening read frame 2184 bp in length and coded a polypeptide of 727 amino acids.The sequence analysis demonstrated that represent reference strains loaded down from GenBank,the result of ORF sequence analysis indicated that CC-1 strain exhibited 99.3% homology of the nucleotides and amino acid with that of the XJ-1 strain in our county.To analyze the sequence of VP1 full gene,have a significance in clinic and epidemiological study.
Keywords:feline parvovirus  VP1 gene  sequencing analysis
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号