Development and Application of EST-SSR Markers of Black Quail |
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Authors: | BAI Jun-yan PANG You-zhi YANG You-bing QI Yan-xia LI Hong-wei |
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Institution: | College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China |
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Abstract: | The purpose of this study was to accelerate the application of molecular markers in genetic breeding and other aspects of Black quail to develop EST-SSR markers for Black quail.100 Black quail were randomly chosen and blood samples were collected to develop EST-SSR new markers and determined the genetic polymorphism using bioinformatics.The results showed that there were 6 EST-SSR markers in the Black quail, the number of observed alleles in EST-SSR was 2 to 6.The average PIC of 6 EST-SSR markers in the Back quail was 0.4962 and the average He was 0.5759.Markers P1 and P2 were highly polymorphic markers among 6 EST-SSR and the rest EST-SSR markers were moderately polymorphic markers.These developed markers could be used for genetic diversity analysis of Black quail and the EST-SSR markers had some relationship with the carbohydrate metabolism, synthesis of melanin in Black quail, but the mechanism needed the further analysis. |
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Keywords: | Black quail EST-SSR bioinformatics genetic diversity |
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