对香豆酸影响猪常温保存精液品质的研究

本试验旨在探讨于猪精液基础稀释液（BTS）中添加不同浓度的对香豆酸（P-coumaric acid，PCA）对猪精液质量及抗氧化酶活性的影响。在猪常温稀释制剂中添加浓度为0、0.03、0.06、0.09、0.12、0.15 g/L的PCA，利用CASA全自动精子分析仪与荧光探针技术分别在1~5 d内检测精子运动学参数与精子功能完整性；利用抗氧化试剂盒在1、3和5 d检测精子活性氧（reactive oxygen species，ROS）和丙二醛（malondialdehyde，MDA）水平以及超氧化物歧化酶（superoxide dismutase，SOD）活性。试验结果表明，在精液保存的第5天，0.09 g/L的PCA处理组与空白组相比，精子活力显著提高（P<0.05），精子的顶体功能完整性、质膜功能完整性、线粒体功能完整性也显著升高（P<0.05），不仅如此，0.03、0.06、0.09和0.12 g/L PCA处理可显著降低精子ROS与MDA水平，提高SOD活性（P<0.05），其中0.09 g/L处理组相比其他组抗氧化活性效果最佳，而当添加浓度为0.15 g/L时，精子的抗氧化活性与0.09 g/L组相比显著降低（P<0.05）。综上，猪常温基础稀释液中添加PCA可改善精子常温保存品质，添加0.09 g/L效果最佳。

Effect of P-coumaric Acid on the Quality of Boar Semen Preserved at Room Temperature

The purpose of this study was to investigate the effects of different concentrations of P-coumaric acid (PCA) added to Beltsville thawing solution (BTS) on boar semen quality and antioxidant enzyme activities.The concentrations of 0, 0.03, 0.06, 0.09, 0.12, 0.15 g/L PCA were added into boar semen dilution preserved at room temperature.The sperm kinetic parameters and sperm functional integrity were detected by CASA automatic sperm analyzer and fluorescence probe technology within 1-5 d.The levels of reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by antioxidant kit on day 1, 3 and 5.The results showed that the sperm motility of the 0.09 g/L PCA group was significantly higher than that of the blank group on the 5th day of semen preservation (P<0.05), the sperm acrosome function integrity, plasma membrane function integrity and mitochondrial function integrity were also significantly higher than blank group (P<0.05).In addition, 0.03, 0.06, 0.09 and 0.12 g/L PCA treatment significantly reduced the levels of ROS and MDA, and increased the activity of SOD (P<0.05).The antioxidant activity of 0.09 g/L PCA, which in comparison with other treatment groups, was won the optimum antioxidant effect.However, when the adding concentration of PCA up to 0.15 g/L, the antioxidant activity of sperm was significantly decreased in comparison with 0.09 g/L (P<0.05).In summary, the addition of 0.09 g/L PCA to BTS could improve the quality of sperm, the best supplementation concentration of PCA was 0.09 g/L.