吉林地区1株小鹅瘟病毒NS1和VP1基因的克隆与序列分析

为了解吉林地区小鹅瘟病毒（Gosling plague virus，GPV）的基因特征，及其与黑龙江及中国其他省份和国外流行毒株的相关性，本研究采用PCR方法鉴定2018年吉林某养鹅场送检的病死雏鹅的肠组织，同时对GPV的非结构蛋白NS1基因及结构蛋白VP1基因进行了克隆和测序，并与国内外16株GPV参考毒株的相应序列进行分析。结果表明，病死雏鹅为GPV与减蛋综合征病毒（Egg drop syndrome virus，EDSV）混合感染；吉林地区GPV的NS1基因长为1 884 bp，编码627个氨基酸，与参考毒株核苷酸序列同源性为93.8%~99.8%，氨基酸序列同源性为97.1%~99.7%；VP1基因长为2 199 bp，编码732个氨基酸，与参考毒株核苷酸序列同源性为93.4%~99.9%，氨基酸序列同源性为96.4%~99.9%。NS1及VP1基因的系统进化树分析均表明，吉林地区GPV与哈尔滨分离株98E属于同一进化分支，亲缘关系最近，与国外分离株、中国台湾和安徽分离株亲缘关系均较远。吉林地区GPV与鹅源GPV具有较近的亲源关系，同源性明显高于其他水禽来源的GPV。该研究为明确中国东北地区GPV空间的流行规律提供基础数据，为东北地区GPV的诊断与治疗提供参考依据。

Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District

In order to understand the genetic characteristics and the correlation of Gosling plague virus(GPV)from Jilin district with isolated strains from Heilongjiang and other provinces of China and abroad,the intestinal tissue of dead goslings from Jilin district in 2018 was detected by PCR.At the same time,the non-structural protein NS1 gene and structural protein VP1 gene of GPV were cloned and sequenced.Sequence analysis were conducted with the corresponding sequence of 16 GPV reference strains of China and abroad.The results showed that the mixed infection of GPV and EDSV was confirmed in dead gosling.The NS1 gene of GPV from Jilin was 1884 bp in length,encoded 627 amino acids,and the nucleotide homology with the reference strains were 93.8%to 99.8%,and the amino acid homology were 97.1%to 99.7%.The VP1 gene of GPV was 2199 bp in length,encoded 732 amino acids,and the nucleotide homology with the reference strains were 93.4%to 99.9%,and the amino acid homology were 96.4%to 99.9%.The phylogenetic tree analysis of NS1 and VP1 genes showed that GPV from Jilin and Harbin isolated 98E strain belonged to the same evolutionary branch,and the relationship was the closest,but far away from abroad,Taiwan and Anhui isolated strains.The NS1 and VP1 amino acid sequence of GPV from Jinlin were analyzed with GPV origined different kinds of waterfowls,the results showed that GPV from Jilin had a closer relationship and the higher homology with goose-origin parvovirus.This study provided basic date for clarifying the spatial epidemic regularity of GPV in Northeast China,and provided a reference basis for the diagnosis and treatment of GPV in Northeast China.