“乳炎宁”水提物的抗炎作用研究

【目的】 探究"乳炎宁"水提物的抗炎作用，为其临床运用及进一步研究提供理论依据。【方法】 采用二甲苯致耳廓肿胀试验、醋酸致腹腔毛细血管通透性试验、角叉菜胶致足肿胀试验、棉球致肉芽肿胀试验及小鼠血清细胞因子含量检测进行"乳炎宁"水提物抗炎作用研究。取60只健康昆明小鼠，雌雄各半，随机分为5组，即阴性对照组(生理盐水组)、阳性对照组(阿司匹林组)、"乳炎宁"水提物低(4 mg/g)、中(8 mg/g)、高(16 mg/g)剂量组，12只/组。各组小鼠每天固定时间灌胃，每天1次，连续10 d。后续按照不同试验具体操作，试验结束后分别测量各组小鼠耳廓重量、腹腔洗液D_{590 nm}值、足重量、棉球肉芽肿重量及血清中肿瘤坏死因子-α(TNF-α)和白介素-1β(IL-1β)含量。【结果】 二甲苯致耳廓肿胀试验结果表明，与阴性对照组相比，药物处理组均可极显著降低二甲苯导致的耳廓肿胀(P<0.01);与阳性对照组相比，"乳炎宁"水提物高剂量组耳廓重量差异不显著(P>0.05)。醋酸致腹腔毛细血管通透性试验结果表明，与阴性对照组相比，药物处理组对醋酸致腹腔毛细血管通透性升高均有极显著抑制作用(P<0.01);与阳性对照组相比，"乳炎宁"水提物各剂量组的毛细血管通透性差异显著或极显著(P<0.05;P<0.01)。角叉菜胶致足肿胀试验结果表明，与阴性对照组相比，药物处理组对小鼠足肿胀度均有极显著的抑制作用(P<0.01);与阳性对照组相比，"乳炎宁"水提物各剂量组足肿胀度均有极显著差异(P<0.01)。棉球致肉芽肿胀试验结果表明，与阴性对照组相比，药物处理组对小鼠棉球肉芽肿胀抑制均有极显著抑制作用(P<0.01);与阳性对照组相比，"乳炎宁"水提物低、中剂量组差异极显著或显著(P<0.01;P<0.05)，"乳炎宁"高剂量组与阳性对照组作用相当(P>0.05)。小鼠血清细胞因子检测实验结果表明，与阴性对照组相比，除"乳炎宁"水提物低剂量组外，药物处理组血清中TNF-α和IL-1β含量均有不同程度降低，"乳炎宁"水提物低剂量组血清中TNF-α含量差异不显著(P>0.05)，血清中IL-1β含量显著降低(P<0.05)，"乳炎宁"水提物中、高剂量组血清中IL-1β含量均极显著降低(P<0.01)，中剂量组血清中TNF-α含量显著降低(P<0.05)，高剂量组TNF-α含量极显著降低(P<0.01);与阳性对照组相比，"乳炎宁"水提物中、高剂量组血清中2种细胞因子均差异不显著(P>0.05)，低剂量组血清中2种细胞因子均差异极显著(P<0.01)。【结论】 "乳炎宁"水提物在小鼠上显示出了良好的抗炎效果。

Study on the Anti-inflammatory Effect of ‘Ruyan Ning’ Water Extract

【Objective】 This study was aimed to explore the anti-inflammatory effect of 'Ruyan Ning’ water extract,and provide theoretical basis for its clinical application and further research.【Method】 The anti-inflammatory effects of xylene-induced auricle swelling,acetic acid-induced increased peritoneal capillary permeability,carrageenan-induced foot swelling,cotton ball-induced granulation swelling,and serum cytokines contents in mice were studied.60 Kunming mice,half male and half female,were randomly divided into 5 groups,namely blank control group (normal saline group),positive control group (aspirin group),and 'Ruyan Ning’ water extract low (4 mg/g),medium (8 mg/g) and high (16 mg/g) dose groups, 12 mice in each group.Mice in each group were intragastrically administered at a fixed time every day,once a day,for 10 consecutive days.Follow-up according to the specific operation of different experiments,after the experiment,the auricle weight,D590 nm value of peritoneal washing fluid,foot weight,cotton ball granuloma weight and serum contents of tumor necrosis factor-α (TNF-α) and interleukin-1β(IL-1β) were measured.【Result】 The results of the xylene-induced auricle swelling test showed that compared with blank control group,the drug treatment group could extremely significantly reduce the auricle swelling caused by xylene (P<0.01).Compared with positive control group,there was no significant difference in auricle weight in the high-dose group (P>0.05).The results of the acetic acid-induced peritoneal capillary permeability test showed that,compared with the blank control group,the drug treatment group had a very significant inhibitory effect on the increase of acetic acid-induced peritoneal capillary permeability (P<0.01).Compared with positive control group,the capillary permeability of each dose group of 'Ruyan Ning’ water extract was significantly or extremely significantly increased (P<0.05;P<0.01).The results of the carrageenan-induced foot swelling test showed that,compared with the blank control group,the drug treatment group had extremely significant inhibitory effect on the foot swelling of mice (P<0.01).Compared with the positive control group,there were extremely significant differences in the degree of foot swelling in each dose group of the 'Ruyan Ning’ water extract (P<0.01).The results of the cotton ball-induced granulation test showed that,compared with the blank control group,the drug treatment group had a very significant inhibitory effect on the inhibition of cotton ball granulation in mice (P<0.01).Compared with the positive control group,the difference between the low and medium dose groups of the 'Ruyan Ning’ water extract was extremely significant or significant (P<0.01;P<0.05),and the high dose group of 'Ruyan Ning’ had the same effect as the positive control group (P>0.05).The results of the detection of serum cytokines in mice showed that compared with the blank control group,except for the low dose 'Ruyan Ning’ water extract group,the contents of TNF-α and IL-1β in serum in the drug treatment group were reduced to varying degrees,'Ruyan Ning’ water extract low dose group had no significant difference in the content of TNF-α in serum (P>0.05),but the content of IL-1β in serum decreased significantly (P<0.05).The content of IL-1β in serum of medium dose group and high dose group were significantly decreased (P<0.01),and the content of TNF-α in serum was significantly decreased in medium dose group (P<0.05),and extremely significantly decreased in high dose group (P<0.01).Compared with the positive control group,there was no significant difference between the two cytokines in serum of the 'Ruyan Ning’ water extract medium and high dose groups (P>0.05),and two cytokines in the serum of the low dose group had extremely significant differences (P<0.01).【Conclusion】 'Ruyan Ning’ water extract had good anti-inflammatory effect on mouse.