| 荧光定量PCR方法的原理及其在研究病毒在动物体内分布规律的应用 |
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| 引用本文： | 高均伟,郭延锋,王三虎,潘耀谦,王丽荣,朱国坡,刘兴友.荧光定量PCR方法的原理及其在研究病毒在动物体内分布规律的应用[J].中国畜牧兽医,2010,37(4):63-65. |
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| 作者姓名： | 高均伟 郭延锋 王三虎 潘耀谦 王丽荣 朱国坡 刘兴友 |
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| 作者单位： | (1.河南科技学院动物科技学院， 新乡 453003； 2.动物疫病和残留物防控河南省高校工程技术研究中心， 新乡 453003) |
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| 基金项目： | 国家自然科学基金(30771601,30972202);;河南省杰出人才创新基金(0521001800);;河南省高校科技创新人才支持计划(2009HASTIT010) |
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| 摘 要： | 研究病毒在动物体内的分布情况,主要是为了掌握病毒在动物体内的生长繁殖规律,找到病毒的靶细胞和靶器官,为进一步研究其致病机理和防制技术提供试验依据。荧光定量PCR法具有定量准确、特异性强、自动化程度高等特点,是研究病毒分布规律的主要方法之一,作者综述了此方法的原理及应用。
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| 关 键 词： | 荧光定量PCR 病毒 分布规律 原理 应用 |
The Principle and Application of Fluorescent Quantitation PCR to Research the Distribution Regulation of Virus in Animal Body |
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| GAO Jun-wei,GUO Yan-feng,WANG San-hu,PAN Yao-qian,WANG Li-rong,ZHU Guo-po,LIU Xing-you.The Principle and Application of Fluorescent Quantitation PCR to Research the Distribution Regulation of Virus in Animal Body[J].China Animal Husbandry & Veterinary Medicine,2010,37(4):63-65. |
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| Authors: | GAO Jun-wei GUO Yan-feng WANG San-hu PAN Yao-qian WANG Li-rong ZHU Guo-po LIU Xing-you |
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| Institution: | （1.School of Animal Science of Henan Institute of Science and Technology, Xinxiang 453003, China；2.Henan Higher Education Engineering Technology Research Centre for Animal Diseases Control and Residues Supervision, Xinxiang 453003, China） |
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| Abstract: | Researching the distribution of virus in the animal body is mainly to understand the regulation of virus growth and multiplication in animal and find the target cell and target organs of virus.Then it can provide the experimental evidence for the mechanism of disease and control technologies.The method of fluorescent quantitative PCR has the characteristic including quantitative accuracy,specificity,and high degree of automation,etc.So it is one of the main ways to research the distribution.The author summa... |
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| Keywords: | fluorescent quantitation PCR virus distribution regulation principle application |
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