三丁酸甘油酯对乙酸刺激仔猪肠道屏障功能的影响

本试验旨在研究三丁酸甘油酯（TB）对乙酸（ACA）刺激仔猪小肠黏膜生长和肠道屏障功能的影响。试验选用18头体重接近、健康的28日龄断奶仔猪（杜×长×大）,随机分为3个处理：对照组、乙酸组和TB组,每个处理6个重复,每个重复1头猪。试验期为21 d。试验期内对照组和乙酸组饲喂基础日粮,TB组饲喂基础日粮+0.1% TB。于试验第15天清晨,乙酸组和TB组仔猪直肠灌注10 mL 10%乙酸,对照组直肠灌注相应剂量的灭菌生理盐水。于试验第18、21天清晨前腔静脉采血,测定血浆二胺氧化酶（DAO）活力;第21天屠宰取样,取空肠、回肠等组织样品,测定仔猪肠道形态结构、肠道黏膜损伤相关基因mRNA水平等指标。结果表明：①未用乙酸刺激前,与对照组相比,日粮中添加0.1% TB对仔猪平均日增重有一定程度提高（P=0.089）,且显著降低了仔猪料重比（P<0.05）;②与对照组相比,乙酸组显著降低了空肠黏膜绒毛高度、回肠黏膜绒毛高度与隐窝深度比值及空肠绒毛表面积（P<0.05）;③与乙酸组相比,TB组显著降低了血浆DAO活力（P<0.05）,极显著降低了回肠AREG基因的相对表达量（P<0.01）。综合上述结果,日粮中添加0.1% TB能缓解乙酸诱导的仔猪肠黏膜生长及肠道屏障功能的损伤。

Effects of Tributyrin on Gut Barrier Function of Acetic Acid-treated Piglets

This study was conducted to study the effect of tributyrin (TB) on small intestine mucosal growth and gut barrier function of acetic acid (ACA)-treated piglets.Eighteen crossbred healthy piglets (Ducoc×Landrace×Yorkshire,28 days old) were randomly allocated into three treatment groups (control,ACA and TB).The pigs in control and ACA groups were fed basal diet,and TB group was fed the basal diet supplemented with 0.1% TB.On day 15 of the trial,ACA and TB groups were intracolonically administrated with 10 mL of 10% ACA solution,control group were given sterile saline at the same volume.On days 18 and 21,blood samples were collected from jugular vein for the determination of DAO activity.On day 21 of the trial,piglets were killed and the samples of small intestinal mucosa were collected for the measuring of intestinal morphological structure and mucosal injury correlated genes mRNA levels.The results showed that:①Before ACA administration,comparing to control group,dietary supplementation with TB could increase ADG,but the difference was not significant (P=0.089),while reduced F/G significantly (P<0.05);②Compared with control group,ACA administration significantly decreased the villus height and surface area of villus in the jejunum,and the ratio of villus height to crypt depth in the ileum (P<0.05);③Compared with ACA group,TB supplementation significantly decreased the plasma DAO activity (P<0.05),and decreased the expression of AREG gene in the ileum mucosa extreme significantly (P<0.01).Collectively,dietary supplementation with 0.1% TB could alleviate ACA-induced damages on mucosal growth and barrier function in the small intestine of piglets.