首页 | 本学科首页   官方微博 | 高级检索  
     检索      

表达猪乳铁蛋白肽重组屎肠球菌对断奶仔猪生长性能的影响及其抗ETEC感染效果研究
引用本文:解伟纯,王玉峰,皮若冰,赵东方,姜艳平,崔文,乔薪瑗,王丽,周晗,徐义刚,李一经,唐丽杰.表达猪乳铁蛋白肽重组屎肠球菌对断奶仔猪生长性能的影响及其抗ETEC感染效果研究[J].中国畜牧兽医,2018,45(12):3408-3418.
作者姓名:解伟纯  王玉峰  皮若冰  赵东方  姜艳平  崔文  乔薪瑗  王丽  周晗  徐义刚  李一经  唐丽杰
作者单位:1. 东北农业大学动物医学学院, 哈尔滨 150030;
2. 东北农业大学食品学院, 哈尔滨 150030
基金项目:国家自然科学基金项目(31672461、30172057);哈尔滨市科技创新人才基金(2014RFXXJ084)
摘    要:本试验以表达猪乳铁蛋白肽的重组屎肠球菌(pNZ8112-PLFcin/Ef)饲喂断奶仔猪,研究其对仔猪生长性能的影响和抗产肠毒素大肠杆菌(enterotoxigenic Escherichia coli,ETEC)感染的效果。选取28日龄体重相近的健康断奶仔猪36头,随机分为3组(重组屎肠球菌组、空载体组和培养基组),每组3个重复,每个重复4头仔猪。重组屎肠球菌组和空载体组分别饲喂添加pNZ8112-PLFcin/Ef (6×1012 CFU/kg)和pNZ8112/Ef (6×1012 CFU/kg)的基础日粮,而培养基组饲喂含有相同体积的GM17液体培养基的基础日粮。试验期26 d。结果显示,与培养基组相比,重组屎肠球菌组断奶仔猪的平均日增重极显著提高(P<0.01);料重比显著降低(P<0.05);腹泻率明显降低,肠道菌群的均匀度和多样性指数均下降。为进一步探究表达猪乳铁蛋白肽的重组屎肠球菌对断奶仔猪抵抗ETEC感染的保护作用,在连续饲喂21 d后,每个重复中随机挑选1头体况相近的断奶仔猪灌服ETEC。结果发现,与培养基组相比,攻菌后重组屎肠球菌组断奶仔猪血清白细胞介素-2(IL-2)、免疫球蛋白G (IgG)含量、肠黏液中分泌型免疫球蛋白A (sIgA)水平均显著升高(P<0.05);脾脏指数显著提高(P<0.05),但胸腺指数、肠段长度及重量则均无显著差异(P>0.05)。综上所述,表达猪乳铁蛋白肽的重组屎肠球菌能够起到促进断奶仔猪生长及抗ETEC感染的保护作用。

关 键 词:猪乳铁蛋白肽  重组屎肠球菌  断乳仔猪  产肠毒素大肠杆菌(ETEC)  
收稿时间:2018-05-15

Effect of Recombinant Enterococcus faecium Expressing Porcine Lactoferrin Peptide on Growth Performances and Its Protection Activity Aganist ETEC of Weaned Piglets
XIE Weichun,WANG Yufeng,PI Ruobing,ZHAO Dongfang,JIANG Yanping,CUI Wen,QIAO Xinyuan,WANG Li,ZHOU Han,XU Yigang,LI Yijing,TANG Lijie.Effect of Recombinant Enterococcus faecium Expressing Porcine Lactoferrin Peptide on Growth Performances and Its Protection Activity Aganist ETEC of Weaned Piglets[J].China Animal Husbandry & Veterinary Medicine,2018,45(12):3408-3418.
Authors:XIE Weichun  WANG Yufeng  PI Ruobing  ZHAO Dongfang  JIANG Yanping  CUI Wen  QIAO Xinyuan  WANG Li  ZHOU Han  XU Yigang  LI Yijing  TANG Lijie
Institution:1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;
2. College of Food Science, Northeast Agricultural University, Harbin 150030, China
Abstract:In this study,the effects of recombinant Enterococcus faecium expressing porcine lactoferrin peptide (pNZ8112-PLFcin/Ef) on growth performance and its protection activity aganist enterotoxigenic Escherichia coli (ETEC) of weaned piglets were examined.36 healthy weaned piglets of 28 days old with similar body weight were selected and randomly divided into 3 groups with 3 replicates per group and 4 piglets per replicate.The piglets in recombinant Enterococcus faecium group was fed the basal diet supplemented with 6×1012 CFU/kg pNZ8112-PLFcin/Ef,that in pNZ8112/Ef group was fed basal diet supplemented with 6×1012 CFU/kg pNZ8112/Ef,while that in the medium group was fed basal diet supplemented with the same volume of GM17 medium.The experiment lasted for 26 d.The results showed that compared with medium group,the average daily gain of weaned piglets in the recombinant Enterococcus faecium group was extremely significantly increased (P<0.01),the F/G was significantly decreased (P<0.05).And the occurrence of piglet diarrhea was reduced to a certain extent.The evenness and diversity indexes of intestinal flora in weaned piglets were decreased.In order to further explore the antibacterial activity of pNZ8112-PLFcin/Ef,the protective effect of ETEC infection was explored in this study.After continuous feeding for 21 days,one piglet randomly selected from each replicate and fed with ETEC.The results showed that compared with the medium group,the levels of IL-2 and IgG in serum,sIgA in intestinal mucus and spleen index of recombinant Enterococcus faecium group were significantly increased (P<0.05),but there was no significant difference in thymus index and the length and weight of small intestinal (P>0.05).In conclusion,the studies had shown that recombinant Enterococcus faecium expressing porcine lactoferrin could promote the growth of piglets and had protective effect of ETEC infection.
Keywords:porcine lactoferricin  recombinant Enterococcus faecium  weaned piglets  enterotoxigenic Escherichia coli(ETEC)  
点击此处可从《中国畜牧兽医》浏览原始摘要信息
点击此处可从《中国畜牧兽医》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号