蛋鸡脂肪肝综合征模型的建立

试验旨在建立蛋鸡脂肪肝综合征病理模型。选取5日龄的海兰褐蛋鸡公雏280只，随机分为对照组（基础日粮组）和3个模型组（A、B、C组），试验第1~10天给模型组饲喂不同配比的高脂饲料，第11~20天饲喂常规基础日粮，每天观察并记录试验鸡精神状态、外观体征、饮水量和食欲情况，试验第0、10、20天从各组随机抽取15只鸡进行翅静脉采血并剖取肝脏和腹脂，测定肝脏系数、肝脂率、腹脂率，血清总胆固醇（TC）、甘油三酯（TG）、谷丙转氨酶（ALT）和谷草转氨酶（AST）水平。结果显示，试验第10天，3个模型组剖检时可见腹腔和肠系膜有大量的脂肪沉积，肝脏系数、肝脂率和腹脂率，以及血清中总胆固醇、甘油三酯、谷丙转氨酶和谷草转氨酶指标均符合鸡脂肪肝综合征模型的诊断标准；试验第20天，模型A、B组谷丙转氨酶和谷草转氨酶水平有所恢复，其他指标仍高于对照组，而模型C组的临床症状和各项检测指标仍符合鸡脂肪肝综合征模型的诊断标准。因此采用连续饲喂高脂饲料C（74.5%基础日粮、6%胆固醇、14%猪油、5%蔗糖、0.5%丙基硫氧嘧啶）可成功建立蛋鸡脂肪肝综合征模型。

Establishment of Fatty Liver Syndrome Model of Egg-type Chickens

This study was aimed to establish the fatty liver syndrome model of egg-type chickens.280 five-day-old Hyline egg-type chickens were selected and randomly divided into control group (basic diet group) and model groups (A,B and C groups).From 0 to 10 days of experiment,the model groups was fed with high-fat diet with different ratio,and the routine basal diet was fed from 11 to 20 days.The experimental chicken mental state,appearance and signs,water intake and appetite of chickens were observed and recorded each day.15 chickens were randomly selected from each group on the 0,10th and 20th days of experiment for blood sampling,liver and abdominal fat extraction.The liver coefficient,hepatic lipid ratio,abdominal fat percentage,serum total cholesterol (TC),triglyceride (TG),alanine transaminase (ALT) and aspartate transaminase (AST) levels were determined.The results showed that there was a lot of fat deposits in the abdominal cavity and mesentery in three modle groups on the 10th day of the experiment,the indicators of liver coefficient,hepatic fat percentage,abdominal fat percentage,serum TC,TG,ALT and AST were accord with the diagnostic criteria of fatty liver syndrome model of chickens.On the 20th day of experiment,the levels of ALT and AST were recovered in model groups A and B,and the other indexes were higher than those in control group,the clinical symptoms and the detection indexes in model group C were accord with the diagnostic criteria of fatty liver syndrome model of chickens.Therefore,continuous feeding of high-fat diet C (74.5% basal diet,6% cholesterol,14% lard,5% sucrose,0.5% propylthiouracil) could successfully establish the fatty liver syndrome model of egg-type chickens.