TGFβ3基因多态位点及其与猪脊椎数性状的关联分析

本研究旨在探索转化生长因子β3（transforming growth factor beta 3，TGFβ3）基因在大白猪×民猪构建的F2代资源群体内的多态性，并分析其多态性与猪脊椎数性状的关系。试验采用PCR技术，以F0代个体的DNA为模板，筛选TGFβ3基因外显子区的SNP，并将筛选到的SNP在F2代群体中进行验证，进而分析SNP与猪脊椎数性状的关联性。结果发现，TGFβ3基因在F0代个体中仅筛选到1个SNP位点，即SSC7：105179474 G > A，表现为两种基因型GG和GA。TGFβ3基因不同基因型与F2代个体脊椎数性状的关联分析表明，SSC7：105179474 G > A与猪肋骨数、胸腰椎数之和呈极显著相关（P < 0.01），与猪腰椎数无显著相关（P > 0.05）。χ²适合性检验发现，该位点处于Hardy-Weinberg平衡状态（P > 0.05）。综上所述，TGFβ3基因可能是影响猪脊椎数性状的主效基因或与主效基因连锁，SSC7：105179474 G > A位点有望作为提高猪脊椎数性状的分子遗传标记。

Polymorphism Sites of TGFβ3 Gene and Its Association Analysis with Vertebral Number of Porcine

This experiment was aimed to investigate single nucleotide polymorphism (SNP) within transforming growth factor beta 3 (TGFβ3) gene and its associations with the vertebral number of porcine(F2 generation resource population of Large White pig×Min pig).We screened the SNP within exons region of TGFβ3 gene in F0 generation individuals using PCR method and verified them in F2 generation,and analyzed its associations with vertebral number of porcine.The result showed that one SNP was found in F0 generation individuals (SSC7:105179474 G > A),which was devised into two genotypes:GG and GA.Association analysis between genotypes and vertebral number of porcine showed that SSC7:105179474 G > A was extremely significantly associated with thoracolumbar number and rib number (P < 0.01),and there was no significant association with the lumber number (P > 0.05).The χ² test demonstrated that this variation was in Hardy-Weinberg equilibrium (P > 0.05).The results demonstrated that TGFβ3 gene was primarily inferred to be a potential major gene or linked to the major gene affecting the vertebral traits of porcine,and SSC7:105179474 G > A might be a candidate molecular genetic markers to improve the vertebral number of porcine.