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| 荷斯坦母牛TLR10基因PCR-SSCP分析 | |
| 引用本文： | 李拓,储明星,王金玉,安永福,狄冉,方丽.荷斯坦母牛TLR10基因PCR-SSCP分析[J].中国畜牧兽医,2009,36(12):81-84. |
| 作者姓名： | 李拓 储明星 王金玉 安永福 狄冉 方丽 |
| 作者单位： | 1. 扬州大学动物科学与技术学院,扬州,225009 2. 中国农业科学院北京畜牧兽医研究所,农业部畜禽遗传资源与利用重点开放实验室,北京,100193 3. 河北省畜牧兽医研究所,保定,071000 |
| 基金项目： | 国家"十一五"科技支撑计划课题，北京市自然科学基金项目 |
| 摘 要： | 设计15对引物,采用PCR-SSCP技术检测Toll样受体10（Toll-like receptor 10,TLR10）基因在210头荷斯坦母牛中的多态性,分析该基因对荷斯坦母牛乳房炎抗性的影响。结果显示,TLR10基因在检测的荷斯坦母牛中都不存在多态性,该基因区域可能不存在影响乳房炎抗性的遗传标记。 |
| 关 键 词： | 荷斯坦母牛 TLR10基因 PCR-SSCP |
PCR-SSCP Analysis on TLR10 Gene in Holstein Cows |
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| LI Tuo,CHU Ming-xing,WANG Jin-yu,AN Yong-fu,DI Ran,FANG Li.PCR-SSCP Analysis on TLR10 Gene in Holstein Cows[J].China Animal Husbandry & Veterinary Medicine,2009,36(12):81-84. | |
| Authors: | LI Tuo CHU Ming-xing WANG Jin-yu AN Yong-fu DI Ran FANG Li |
| Institution: | LI Tuo1,CHU Ming-xing2,WANG Jin-yu1,AN Yong-fu3,DI Ran2,FANG Li2(1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China,2.Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,3.Hebei Animal Science and Veterinary Medicine Institute,Baoding 071000,China) |
| Abstract: | The TLR10(Toll-like receptor 10) gene was studied as a candidate gene for mastitis resistance in 210 Holstein cows.Polymorphism of TLR10 gene was detected with fifteen pairs of primers by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) in 210 Holstein cows.No polymorphism was detected in the amplified region,and this result indicated that the genetic markers that affect mastitis resistance in Holstein cows may not exist in the detected region of TLR10 gene. |
| Keywords: | PCR-SSCP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |