首页 | 本学科首页   官方微博 | 高级检索  
     检索      

小鼠肝炎病毒金标快速检测方法的建立
引用本文:胡晓燕,佟巍,刘先菊,张丽芳,刘云波.小鼠肝炎病毒金标快速检测方法的建立[J].中国畜牧兽医,2011,38(7):96-100.
作者姓名:胡晓燕  佟巍  刘先菊  张丽芳  刘云波
作者单位:中国医学科学院北京协和医学院,医学实验动物研究所,北京 100021
基金项目:中央级公益性科研院所基本科研业务费专项资金项目
摘    要:本试验旨在探讨一种能够快速、简便及特异检测MHV病毒的金标检测方法。采用柠檬酸三钠还原法制备胶体金颗粒,标记抗小鼠肝炎病毒(MHV)抗体,选择优化层析条件,建立双抗体夹心模式的免疫层析法试纸条对MHV进行检测。20 nm左右的胶体金颗粒、5.05 μg为最低稳定抗体量(稳定0.5 mL胶体金)、M135层析材料、3%BSA是最佳封闭液。640 μg/mL抗体蛋白为最适检测线包被浓度,而800 μg/mL抗体蛋白为质控线最适包被浓度。此试纸条能10 min快速特异地检测出MHV抗原,阳性时检测线和质控线均显示红色,阴性时则只有质控线显示红色。结果表明,胶体金免疫层析方法能快速、特异、稳定地检测MHV抗原。

关 键 词:小鼠肝炎病毒  抗小鼠肝炎病毒抗体  胶体金  胶体金免疫层析法  

Development of a MHV Immune Colloidal Gold Strip
HU Xiao-yan,TONG Wei,LIU Xian-ju,ZHANG Li-fang,LIU Yun-bo.Development of a MHV Immune Colloidal Gold Strip[J].China Animal Husbandry & Veterinary Medicine,2011,38(7):96-100.
Authors:HU Xiao-yan  TONG Wei  LIU Xian-ju  ZHANG Li-fang  LIU Yun-bo
Institution:Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
Abstract:To develop a quick, simple and specific method for the detection of MHV which cause hepatitis of mice,colloidal gold was prepared by reduction of HAuCl4 with trisodium citrate and label with purified MHV-Ab against MHV. The qualification and conditions of GICAs were selected and optimized. So the GICA method was built to detect MHV. Colloidal gold with about 20 nm particle diameter, the least amount of antibody to stabilize the 0.5 mL colloidal gold is 5.05 μg. We chose M135 as the chromatography material, and 3% BSA as the confining liquid. The most appropriate amount of test line antibody protein is 640 μg/mL, and the amount of quality control line antibody protein is 800 μg/mL. The method could detect the MHV about 10 minutes with specificity. While positive result, the test line and the quality control line were red. As for negative result, only the quality control line was red. The method of GICA in this paper could detect the MHV rapidly with specificity and stability.
Keywords:MHV  MHV-Ab  colloidal gold  GICA
本文献已被 万方数据 等数据库收录!
点击此处可从《中国畜牧兽医》浏览原始摘要信息
点击此处可从《中国畜牧兽医》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号