| 羊布氏杆菌的分离与鉴定 |
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| 引用本文： | 欧燎原,谈志祥,何世成,黄建龙,张朝阳,鲁杏华,唐小明,汪洪冰.羊布氏杆菌的分离与鉴定[J].中国畜牧兽医,2011,38(8):231-233. |
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| 作者姓名： | 欧燎原 谈志祥 何世成 黄建龙 张朝阳 鲁杏华 唐小明 汪洪冰 |
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| 作者单位： | 湖南省动物疫病预防控制中心,湖南长沙 410007 |
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| 摘 要： | 从一患睾丸炎公山羊中分离到一株革兰氏阴性短小杆菌,该菌经柯兹洛夫斯基鉴别染色红色,在普通绵羊血琼脂、巧克力平板上生长缓慢,对该菌的16S rDNA进行PCR扩增,测序,BLAST比较,结果显示该菌与羊布氏杆菌的16S rDNA有100%的同源性,菌体与布氏杆菌标准阳性血清起强凝集反应。结果显示,该分离菌为布氏杆菌。
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| 关 键 词： | 布氏杆菌 16S rDNA PCR 鉴定 |
Isolation and Identification of Brucella melitensis |
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| OU Liao-yuan,TAN Zhi-xiang,HE Shi-cheng,HUANG Jian-long,ZHANG Chao-yang,LU Xing-hua,TANG Xiao-ming,WANG Hong-bing.Isolation and Identification of Brucella melitensis[J].China Animal Husbandry & Veterinary Medicine,2011,38(8):231-233. |
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| Authors: | OU Liao-yuan TAN Zhi-xiang HE Shi-cheng HUANG Jian-long ZHANG Chao-yang LU Xing-hua TANG Xiao-ming WANG Hong-bing |
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| Institution: | Animal Disease Control Center of Hunan Province,Changsha 410007,China |
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| Abstract: | A gram negative Bacillus pumilus were isolated from a orchiditis goat, it was red in differential staining Coates Pavlovsky, and grew slowly in sheep’s blood agar and chocolate agar. Its 16S rDNA sequence amplification by PCR was 100% identities with Brucella melitensis in GenBank by BLAST, and was strong positive in the agglutination test between the isolated bacterium with the reference Brucella’s antiserum. The two test results shows the isolated bacterium were Brucella melitensis. |
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| Keywords: | Brucella 16S rDNA PCR identification |
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