| 细胞松弛素B对牛GV期卵母细胞玻璃化冷冻效果的影响 |
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| 引用本文： | 郭娟,王志琴,赵云程,林嘉鹏,黄俊成.细胞松弛素B对牛GV期卵母细胞玻璃化冷冻效果的影响[J].中国畜牧兽医,2009,36(9):101-103. |
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| 作者姓名： | 郭娟 王志琴 赵云程 林嘉鹏 黄俊成 |
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| 作者单位： | （1.新疆农业大学动物医学学院， 乌鲁木齐 830052； 2.新疆畜牧科学院农业部家畜繁育生物技术重点开放实验室， 乌鲁木齐 830000) |
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| 基金项目： | 新疆高技术研究与发展项目，基金项目，国家863项目 |
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| 摘 要： | 本试验通过玻璃化冷冻前不同浓度细胞松弛素B(cytochalasin-B,CB)预处理来分析CB对牛GV期卵母细胞固体表面玻璃化(SSV)冷冻后发育潜力的影响。试验结果表明，玻璃化冷冻后，CB处理组和未处理组之间卵母细胞成熟率差异不显著(P>0.05)，但均显著低于体外培养组。说明试验中所用CB浓度 (2.5、7.5、15、20和30 μg/mL)对牛GV期卵母细胞玻璃化冷冻效果的改善作用不明显。
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| 关 键 词： | 玻璃化冷冻 细胞松弛素B 牛 GV期卵母细胞 |
Effects of Cytochalasin B on Vitrification of Bovine GV-stage Oocytes |
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| GUO Juan,WANG Zhi-qin,ZHAO Yun-cheng,LIN Jia-peng,HUANG Jun-cheng.Effects of Cytochalasin B on Vitrification of Bovine GV-stage Oocytes[J].China Animal Husbandry & Veterinary Medicine,2009,36(9):101-103. |
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| Authors: | GUO Juan WANG Zhi-qin ZHAO Yun-cheng LIN Jia-peng HUANG Jun-cheng |
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| Institution: | (1.Department of Veterinary Medicine，Xinjiang Agriculture University， Urumqi 830052，China; 2.The Key Laboratoy of Animal Biotechnology of Ministry of Agriculture， Xinjiang Academy of Animal Sciences，Urumqi 830000,China) |
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| Abstract: | In the present study， effects of concentration of cytochalasin B(CB) on solid surface vitrification (SSV) of bovine GV-stage oocytes were examined. The results demonstrated that no significant differences were observed in vitro maturation rate among with or without CB pre-treatment，while they were all significantly lower than that in the IVM group.In conclusion，different concentration CB(2.5,7.5,15,20 and 30 μg/mL)treatment before vitrification did not improve the IVM rate following the vitrification of immature bovine oocytes. |
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| Keywords: | vitrification cytochalasin B bovine GV-stage oocytes |
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