哺乳动物精子性别鉴定

性别鉴定技术与人们的日常生活及畜牧业的发展有着密切的关系。精子性别鉴定技术的迅速发展为这一技术的应用提供了广阔的前景。目前最常用的精子性别鉴定技术是流式细胞分选法 ,此法依据 X-精子及 Y-精子 DNA含量的不同将两种精子分开 ,还可以用作分类后精子的检验。因流式细胞分选法存在一些不适于大规模应用的缺陷 ,人们希望能依据分类后的精子之间的对比研究找出特异的蛋白制成抗体 ,设计出高效免疫的精子性别鉴定技术 ,关于这方面的研究已经取得了一定的进展

Sexing Mammalian Sperm

In people's daily life and the progress of stock raising,Sexing technology has an important application.The development of sexing sperm technology provides a bright future for this technology.Currently,the only successful method for separating X and Y-chromosome-bearing sperm is flow cytometry.The separation of X and Y-chromosome-bearing sperm bases on the difference in their DNA contents.The method is also used to analyze the sperm following separation.Since the procedure of flow cytometry has many disadvantages,such as small production,potentially invasive nature,an alternative would be to identify an immunological marker confined to one sperm type.The present results suggest that a sexing procedure of the viable and immunological sperm can be developed.