| 鸡源大肠杆菌AmpC型β-内酰胺酶的分子检测 |
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| 引用本文： | 刘河冰,李新生,张素梅,莫娟,肖方,潘玉善,杜向党.鸡源大肠杆菌AmpC型β-内酰胺酶的分子检测[J].中国畜牧兽医,2009,36(9):34-36. |
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| 作者姓名： | 刘河冰 李新生 张素梅 莫娟 肖方 潘玉善 杜向党 |
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| 作者单位： | （河南农业大学牧医工程学院， 郑州 450002） |
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| 基金项目： | 河南省青年骨干教师资助计划;"十一五"国家科技支撑计划 |
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| 摘 要： | 为了解河南地区AmpC型β-内酰胺酶耐药基因在产16S rRNA甲基化酶鸡大肠杆菌的流行与分布，分别设计特异性引物对2005—2008年在河南地区病鸡的病料中分离保存的158株16S rRNA甲基化酶阳性鸡大肠杆菌进行聚合酶链式反应扩增。检测结果显示，在158株产16S rRNA甲基化酶鸡大肠杆菌中，CMY-2和DHA-1检出率分别为34.8%和36.1%。提示AmpC型β-内酰胺酶耐药基因在河南地区产16S rRNA甲基化酶鸡大肠杆菌中普遍存在。
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| 关 键 词： | 大肠杆菌 AmpC β-内酰胺酶 耐药 |
Prevalence of AmpC-type β-lactamase among 16S rRNA Methylase-producing Escherichia coli Strains Isolated from Diseased Chickens |
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| LIU He-bing,LI Xin-sheng,ZHANG Su-mei,MO Juan,XIAO Fang,PAN Yu-shan,DU Xiang-dang.Prevalence of AmpC-type β-lactamase among 16S rRNA Methylase-producing Escherichia coli Strains Isolated from Diseased Chickens[J].China Animal Husbandry & Veterinary Medicine,2009,36(9):34-36. |
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| Authors: | LIU He-bing LI Xin-sheng ZHANG Su-mei MO Juan XIAO Fang PAN Yu-shan DU Xiang-dang |
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| Institution: | （College of Animal Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China） |
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| Abstract: | In order to investigate the prevalence of AmpC-type β-lactamase among 16S rRNA methylase-producing Escherichia coli strains isolated from diseased chickens in Henan province,158 16S rRNA methylase-producing E. coli strains isolated from diseased chickens during the years 2005 to 2008 were subjected to PCR for the presence of AmpC-type β-lactamase. The results revealed that of 158 16S rRNA methylase-producing E. coli strains, the positive rates of CMY-2, DHA-1 were 34.8% and 36.1%, respectively, suggesting AmpC-type β-lactmase were prevalent among 16S rRNA methylase-producing E. coli strains isolated from diseased chickens in Henan province. |
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| Keywords: | AmpC |
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