| 富含ω-3多不饱和脂肪酸克隆猪制备的关键技术研究 |
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| 引用本文： | 潘登科,陈红星,冯书堂,李奎,邓继先.富含ω-3多不饱和脂肪酸克隆猪制备的关键技术研究[J].中国畜牧兽医,2007,34(5):22-25. |
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| 作者姓名： | 潘登科 陈红星 冯书堂 李奎 邓继先 |
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| 作者单位： | 1.中国农业科学院北京畜牧兽医研究所基因与细胞工程室, 北京 100094； 2.军事医学科学院生物工程研究所，北京 100071 |
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| 基金项目： | 国家高技术研究发展计划(863计划);国家科技攻关项目;国家自然科学基金 |
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| 摘 要： | 作者综述了制备富含ω-3多不饱和脂肪酸克隆猪的研究意义和国内外的研究现状，并介绍了自己的研究进展。用化学合成的方法获得了C briggsae的ω-3脂肪酸去饱和酶基因sFat-1，并构建了哺乳动物细胞表达载体。通过显微注射的方法制备了转基因小鼠，sFat-1基因能够在转基因小鼠中产生更多更长链的而且更有价值的DHA以及DPA。在确认sFat-1基因功能的基础上，将载体转染到猪胎儿成纤维细胞，利用阳性的细胞克隆通过核移植方式制备富含ω-3多不饱和脂肪酸克隆猪。
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| 关 键 词： | ω-3脂肪酸去饱和酶基因 多不饱和脂肪酸 sFat1 转基因小鼠 克隆猪 |
| 文章编号： | 1671-7236（2007）05-0022-04 |
| 修稿时间： | 2007-04-05 |
Recent Research Advances of Cloning Transgenic Pigs Rich in Omega-3 Fatty Acids |
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| PAN Deng-ke,CHENG Hong-xing,FENG Shu-tang,LI Kui,DENG Ji-xian.Recent Research Advances of Cloning Transgenic Pigs Rich in Omega-3 Fatty Acids[J].China Animal Husbandry & Veterinary Medicine,2007,34(5):22-25. |
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| Authors: | PAN Deng-ke CHENG Hong-xing FENG Shu-tang LI Kui DENG Ji-xian |
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| Institution: | 1.Department of Gene and Cell Engineering, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2. Institute of Biotechnology，Academy of Military Medical Sciences, Beijing 100071, China |
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| Abstract: | In this paper, the significance and rencent progress of transgenic pigs rich in omega-3 fatty acids are presented. In our study，the sFat-1 gene was chemically synthesized according to the sequence from Caenorhabditis briggssae，and constructed into a mammal expression vector pcDNA3.1 sFat-l EGFP. Transgenic sFat-1 gene mice were generated by microinjection of vector into pronuclear zygotes. A modified n-3 fatty acid desaturase gene from C. Briggsae produced high proportion of DHA and DPA in transgenic mice. Based on Function Analysis of n-3 Fatty Acid Desaturase Gene sFat-1, porcine fetal fibroblasts were transfected with vector pcDNA3.1 sFat-l EGFP. Positive colony was chosed as donors for nuclear transfer to enucleated MⅡoocytes, cloning transgenic pigs rich in omega-3 fatty acids. |
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| Keywords: | sFat1 |
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